The natural sequence of events in larval settlement and metamorphosis of Hydroides elegans (Polychaeta; Serpulidae).
The natural sequence of events in larval settlement and metamorphosis of Hydroides elegans (Polychaeta; Serpulidae).
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DOI：
10.1371/journal.pone.0249692
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Nedved BT
中科院分区：
文献类型：
--
作者：
Hadfield MG;Freckelton ML;Nedved BT
广泛分布的秀丽隐杆线虫Hydroides elegans已成为研究海洋生物污损、发育以及由表面微生物膜诱导的幼虫沉降和变态过程的模式生物。对比这些招募过程的初始事件的描述，无论定居是由（1）天然多物种生物膜，（2）由已知诱导定居的单一细菌物种组成的生物膜，还是（3）细菌提取物刺激的研究。我们发现，自然的生物膜或生物膜形成的细菌Pseudoalteromonas luteoviolacea诱导的解决总是发起附着和分泌的粘附和幼虫包裹的初级管，然后由运动纤毛和纤毛细胞和形态发生的损失。含有复杂的tailocin阵列的细菌提取物衍生自噬菌体基因的组合纳入细菌基因组似乎诱导解决事件的幼虫纤毛和纤毛细胞的破坏，然后由附件和初级管的形成。类似的破坏也发生在H.将裸鳃亚目腹足动物的秀丽隐翅虫或幼虫暴露于提取物，尽管它们都没有变态。我们认为，幼虫失去纤毛附着前将被冲走的网站，刺激解决的湍流特性的大多数海洋栖息地。
The broadly distributed serpulid worm Hydroides elegans has become a model organism for studies of marine biofouling, development and the processes of larval settlement and metamorphosis induced by surface microbial films. Contrasting descriptions of the initial events of these recruitment processes, whether settlement is induced by (1) natural multi-species biofilms, (2) biofilms composed of single bacterial species known to induce settlement, or (3) a bacterial extract stimulated the research described here. We found that settlement induced by natural biofilms or biofilms formed by the bacterium Pseudoalteromonas luteoviolacea is invariably initiated by attachment and secretion of an adherent and larva-enveloping primary tube, followed by loss of motile cilia and ciliated cells and morphogenesis. The bacterial extract containing complex tailocin arrays derived from an assemblage of phage genes incorporated into the bacterial genome appears to induce settlement events by destruction of larval cilia and ciliated cells, followed by attachment and primary-tube formation. Similar destruction occurred when precompetent larvae of H. elegans or larvae of a nudibranch gastropod were exposed to the extract, although neither of them metamorphosed. We argue that larvae that lose their cilia before attachment would be swept away from the sites that stimulated settlement by the turbulent flow characteristic of most marine habitats.
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影响因子：
5.2
作者：
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通讯作者：
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影响因子：
2.4
作者：
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影响因子：
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通讯作者：
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