The Phosphoproteomic Response of Okra (Abelmoschus esculentus L.) Seedlings to Salt Stress
The Phosphoproteomic Response of Okra (Abelmoschus esculentus L.) Seedlings to Salt Stress
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黄秋葵幼苗对盐胁迫的磷酸化蛋白质组响应
DOI：
10.3390/ijms20061262
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发表时间：
2019-03
影响因子：
5.6
通讯作者：
Zhan Yihua
中科院分区：
文献类型：
--
作者：
Yu Chenliang;Wu Qinqfei;Sun Chendong;Tang Mengling;Sun Junwei;Zhan Yihua
土壤盐渍化是严重威胁土地利用效率和作物产量的主要环境胁迫。虽然植物对NaCl的整体反应已经得到了很好的研究，但蛋白质磷酸化在黄秋葵（Abelmoschus esculentus L.）苗不清楚。本文探讨了黄秋葵幼苗对300 mM NaCl胁迫响应的分子基础。采用亲和富集、串联质量标签（TMT）标记和高效液相色谱-串联质谱分析相结合的方法，对黄秋葵进行了大规模的磷酸化蛋白质组分析。在2550个蛋白质上共鉴定出4341个磷酸化位点，其中2268个蛋白质的3453个位点提供了定量信息。我们发现，在NaCl/对照比较组中，91个位点上调，307个位点下调。随后，我们进行了系统的生物信息学分析，包括基因本体注释，结构域注释，亚细胞定位和京都基因百科全书和基因组通路注释。后者揭示了差异表达的蛋白质与“光合作用天线蛋白”和“RNA降解”的关系最密切。这些差异表达蛋白可能在黄秋葵盐胁迫响应中发挥重要作用。这些结果有助于我们进一步了解植物在盐胁迫下的翻译后修饰的分子机制。
Soil salinization is a major environmental stresses that seriously threatens land use efficiency and crop yields worldwide. Although the overall response of plants to NaCl has been well studied, the contribution of protein phosphorylation to the detoxification and tolerance of NaCl in okra (Abelmoschus esculentus L.) seedlings is unclear. The molecular bases of okra seedlings’ responses to 300 mM NaCl stress are discussed in this study. Using a combination of affinity enrichment, tandem mass tag (TMT) labeling and high-performance liquid chromatography–tandem mass spectrometry analysis, a large-scale phosphoproteome analysis was performed in okra. A total of 4341 phosphorylation sites were identified on 2550 proteins, of which 3453 sites of 2268 proteins provided quantitative information. We found that 91 sites were upregulated and 307 sites were downregulated in the NaCl/control comparison group. Subsequently, we performed a systematic bioinformatics analysis including gene ontology annotation, domain annotation, subcellular localization, and Kyoto Encyclopedia of Genes and Genomes pathway annotation. The latter revealed that the differentially expressed proteins were most strongly associated with ‘photosynthesis antenna proteins’ and ‘RNA degradation’. These differentially expressed proteins probably play important roles in salt stress responses in okra. The results should help to increase our understanding of the molecular mechanisms of plant post-translational modifications in response to salt stress.
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DOI：
10.1105/tpc.13.6.1467
发表时间：
2001-06
期刊：
The Plant cell
影响因子：
--
作者：
S. Peck;T. Nühse;Daniel Hess;Alejandro Iglesias;Frederick Meins;Thomas Boller
通讯作者：
S. Peck;T. Nühse;Daniel Hess;Alejandro Iglesias;Frederick Meins;Thomas Boller
使用串联质量标签标记对不同颜色稳定性的肌肉进行定量磷酸蛋白质组学分析
影响因子：
8.8
作者：
Li Zheng;Li Meng;Li Xin;Xin Jianzeng;Wang Ying;Shen Qingwu W;Zhang Dequan
通讯作者：
Zhang Dequan
比较蛋白质组学分析提供了对参与黄瓜 (Cucumis sativus L.) 涝胁迫下不定根萌发的关键蛋白质的见解
影响因子：
5.6
作者：
Xu X;Ji J;Ma X;Xu Q;Qi X;Chen X
通讯作者：
Chen X
定量磷酸化蛋白质组分析有助于深入了解沙冬青根部对短期干旱胁迫的反应。
影响因子：
5.6
作者：
Sun Huigai;Xia Bolin;Wang Xue;Gao Fei;Zhou Yijun
通讯作者：
Zhou Yijun
影响因子：
5.6
作者：
Maeda H;Song W;Sage T;Dellapenna D
通讯作者：
Dellapenna D