Enantioseparation of (DL)-tryptophan by spiral tube assembly counter-current chromatography and evaluation of mass transfer rate for enantiomers.
Enantioseparation of (DL)-tryptophan by spiral tube assembly counter-current chromatography and evaluation of mass transfer rate for enantiomers.
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DOI：
10.1016/j.chroma.2014.11.013
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发表时间：
2014-12-29
期刊：
影响因子：
--
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Tong S;Ito Y;Ma Y
以牛血清白蛋白为手性选择剂，将螺旋管组装逆流色谱成功地应用于DL-色氨酸对映体的分离。采用改进的两相水-水溶剂体系12.0%（w/w）聚乙二醇8000-9.0%（w/w）磷酸氢二钾-0.1%氨水-78.9%水作为逆流色谱的溶剂体系，其中牛血清白蛋白主要分布在两相水体系的下相。在水-水溶剂体系中，D-和L-色氨酸的对映选择性均很高，α=2.605沿着分配比DD=1.200，DL=0.461。在室温下，用螺旋管逆流色谱法对2.0 mg DL-色氨酸进行分离，得到了高峰分离度。结果表明，在水-水溶剂体系中加入0.1%的氨水可大大改善对映体的分离。在对映体分离期间观察到L-色氨酸的不寻常的极宽峰。为了给出解释，测量了D-和L-对映体通过两相之间的界面的传质速率。结果表明，L-色氨酸的传质速率低于D-色氨酸。对不同对映体间传质速率差异的可能原因进行了讨论。
Spiral tube assembly counter-current chromatography was successfully applied in enantioseparation of DL-tryptophan using bovine serum albumin as chiral selector. An improved biphasic aqueous-aqueous solvent system 12.0% (w/w) polyethyleneglycol 8000–9.0% (w/w) dibasic potassium phosphate-0.1% ammonia-78.9% water was used as the solvent system for counter-current chromatography, in which bovine serum albumin was predominantly distributed in the lower phase of the two-phase aqueous system. The aqueous-aqueous solvent system gave a very high enantioselectivity for D- and L-tryptophan at α=2.605 along with distribution ratio DD=1.200 and DL=0.461. High peak resolution was obtained for enantioseparation of 2.0 mg of DL-tryptophan by spiral tube assembly counter-current chromatography under room temperature. It was found that 0.1% ammonia added in the aqueous-aqueous solvent system greatly improved the enantioseparations. An unusual extremely broad peak for L-tryptophan was observed during enantioseparations. In order to give an explanation, mass transfer rates of D- and L-enantiomers through the interface between the two phases were measured. It was found that L-tryptophan showed lower mass transfer rate than D-tryptophan. Further discussions were proposed for possible reasons for mass transfer rate difference between the enantiomers.
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影响因子：
4.1
作者：
Tong, Shengqiang;Yan, Jizhong;Lu, Youming
通讯作者：
Lu, Youming
DOI：
10.1080/10826070802019913
发表时间：
2008-01-01
影响因子：
1.3
作者：
Ito, Y.;Clary, R.;Finn, T. M.
通讯作者：
Finn, T. M.
影响因子：
4.1
作者：
Ito, Y;Matsuda, K;Qi, L
通讯作者：
Qi, L
DOI：
10.1016/s0021-9673(00)96187-9
发表时间：
1984-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
ALLENMARK, S;BOMGREN, B;BOREN, H
通讯作者：
BOREN, H
影响因子：
1.3
作者：
Tao, WA;Gilpin, RK
通讯作者：
Gilpin, RK