Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids.
Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids.
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DOI：
10.1016/j.ymben.2013.12.003
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发表时间：
2014-03
影响因子：
8.4
通讯作者：
Sayanova, Olga
中科院分区：
文献类型：
--
作者：
Hamilton, Mary L.;Haslam, Richard P.;Napier, Johnathan A.;Sayanova, Olga
我们已经改造了三角褐指藻来积累高价值的omega-3长链多不饱和脂肪酸二十二碳六烯酸(DHA)。这是通过产生转基因菌株来实现的，在转基因菌株中表达了来自牛牛链球菌的Δ5-伸长酶，以增强内源脂肪酸的生物合成途径。异源伸长酶的表达导致二十二碳六烯酸含量增加了八倍，这代表着这种微藻的脂肪酸组成发生了显著而有价值的变化。重要的是，DHA在三酰甘油中积累，在转基因菌株中检测到几种新的三酰甘油物种。在第二次迭代中，依赖于酰辅酶A的Δ6-去饱和酶和Δ5-拉伸酶的共表达进一步增加了DHA值。总而言之，这首次展示了使用迭代代谢工程优化藻类中omega-3含量的潜力。在微藻中利用代谢工程修饰高价值omega-3长链多不饱和脂肪酸积累的第一个例子。第一个在硅藻中表达多基因的例子。三角褐指藻硅藻的详细脂肪组学分析。
We have engineered the diatom Phaeodactylum tricornutum to accumulate the high value omega-3 long chain polyunsaturated fatty acid docosahexaenoic acid (DHA). This was achieved by the generation of transgenic strains in which the Δ5-elongase from the picoalga Ostreococcus tauri was expressed to augment the endogenous fatty acid biosynthetic pathway. Expression of the heterologous elongase resulted in an eight-fold increase in docosahexaenoic acid content, representing a marked and valuable change in the fatty acid profile of this microalga. Importantly, DHA was shown to accumulate in triacylglycerols, with several novel triacylglycerol species being detected in the transgenic strains. In a second iteration, co-expression of an acyl-CoA-dependent Δ6-desaturase with the Δ5-elongase further increased DHA levels. Together, this demonstrates for the first time the potential of using iterative metabolic engineering to optimise omega-3 content in algae. First example of using metabolic engineering in microalgae to modify the accumulation of high value omega-3 long chain polyunsaturated fatty acids. First example of multigene expression in a diatom. Detailed lipidomic analysis of diatom Phaeodactylum tricornutum.
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影响因子：
1.9
作者：
Calder PC
通讯作者：
Calder PC
影响因子：
6.5
作者：
Meyer, A;Kirsch, H;Heinz, E
通讯作者：
Heinz, E
影响因子：
2.9
作者：
HARRISON, PJ;WATERS, RE;TAYLOR, FJR
通讯作者：
TAYLOR, FJR
影响因子：
8.4
作者：
Ruiz-Lopez, Noemi;Haslam, Richard P.;Usher, Sarah L.;Napier, Johnathan A.;Sayanova, Olga
通讯作者：
Sayanova, Olga
影响因子：
4.1
作者：
Domergue, F;Abbadi, A;Heinz, E
通讯作者：
Heinz, E