Mosquito and human hepatocyte infections with Plasmodium ovale curtisi and Plasmodium ovale wallikeri.
Mosquito and human hepatocyte infections with Plasmodium ovale curtisi and Plasmodium ovale wallikeri.
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卵形疟原虫柯蒂西疟原虫和卵形疟原虫沃里克氏疟原虫引起的蚊子和人类肝细胞感染。
DOI：
10.1093/trstmh/trz048
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发表时间：
2019
影响因子：
2.2
通讯作者：
Kristan M
中科院分区：
文献类型：
--
作者：
Kristan M
背景人类卵形疟疾是由两个亲缘关系密切的物种--卵形疟原虫和恶性疟原虫引起的。瓦利克里卵圆形。已知这两个物种在最初感染发生后数月或数年内从静止的肝脏形态中复发。虽然一些研究已经成功地建立了卵形疟疾的蚊子传播，但还没有专门描述这两个兄弟物种的传播和人类肝细胞感染的情况。卵形曲线和P。利用敏感的寄生虫DNA检测，通过环激活扩增，在吸血蚊子中检测卵形似人按蚊和简化感染监测。卵形屈曲，1个P。从蚊子唾液腺中分离出活子孢子，并成功地感染培养的人肝细胞。结论本方法为利用EDTA采集的卵形疟疾患者的前临床血液样本进行蚊虫感染研究和肝脏OFP生命周期阶段的产生提供了一种方法。卵形曲线和P。我们还证明了环路激活扩增作为一种快速和灵敏的替代解剖的方法来评估以疟原虫感染的血液喂养的按蚊的感染流行率。
BackgroundHuman ovale malaria is caused by the two closely related species,Plasmodium ovale curtisiandP. ovale wallikeri. Both species are known to relapse from quiescent hepatic forms months or years after the primary infection occurred. Although some studies have succeeded in establishing mosquito transmission for ovale malaria, none have specifically described transmission and human hepatocyte infection of both sibling species.MethodsHere we describe a simplified protocol for successful transmission of bothP. ovale curtisiandP. ovale wallikeritoAnopheles coluzziimosquitoes and streamlined monitoring of infection using sensitive parasite DNA detection, by loop-activated amplification, in blood-fed mosquitoes.ResultsIn one experimental infection withP. ovale curtisiand one withP. ovale wallikeri, viable sporozoites were isolated from mosquito salivary glands and used to successfully infect cultured human hepatocytes.ConclusionsThis protocol provides a method for the utilisation of pretreatment clinical blood samples from ovale malaria patients, collected in EDTA, for mosquito infection studies and generation of the hepatic life cycle stages ofP. ovale curtisiandP. ovale wallikeri.We also demonstrate the utility of loop-activated amplification as a rapid and sensitive alternative to dissection for estimating the prevalence of infection inAnophelesmosquitoes fed withPlasmodium-infected blood.
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DOI：
10.1016/0035-9203(76)90132-2
发表时间：
1976-01-01
影响因子：
2.2
作者：
SHUTE, PG;LUPASCU, G;GARNHAM, PCC
通讯作者：
GARNHAM, PCC
影响因子：
9.6
作者：
Ferguson, HM;Read, AF
通讯作者：
Read, AF
DOI：
10.1093/jac/dky297
发表时间：
2018-11-01
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Chairat K;Jittamala P;Hanboonkunupakarn B;Pukrittayakamee S;Hanpithakpong W;Blessborn D;White NJ;Day NPJ;Tarning J
通讯作者：
Tarning J
DOI：
10.4269/ajtmh.2007.77.242
发表时间：
2007-08-01
影响因子：
3.3
作者：
Solarte, Yezid;Manzano, Maria del Rosario;Arevalo-Herrera, Myriam
通讯作者：
Arevalo-Herrera, Myriam
冷冻保存的临床分离株中的间日疟原虫对实验室按蚊群体的感染。
影响因子：
4
作者：
Shaw-Saliba,Kathryn;Clarke,David;Santos,JorgeM;Menezes,MariaJosé;Lim,Caeul;Mascarenhas,Anjali;Chery,Laura;Gomes,Edwin;March,Sandra;Bhatia,SangeetaN;Rathod,PradipsinhK;Ferreira,MarceloU;Catteruccia,Flaminia;Duraisingh,Manoj
通讯作者：
Duraisingh,Manoj