iTRAQ-based quantitative proteomic analysis of thoracic aortas from adult rats born to preeclamptic dams.
iTRAQ-based quantitative proteomic analysis of thoracic aortas from adult rats born to preeclamptic dams.
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基于iTRAQ技术对子痫前期母鼠所产成年子代大鼠胸主动脉的定量蛋白质组学分析
DOI：
10.1186/s12014-021-09327-9
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发表时间：
2021-08-21
影响因子：
3.8
通讯作者：
Pan HT
中科院分区：
文献类型：
--
作者：
Yu B;Zhu HD;Shi XL;Chen PP;Sun XM;Xia GY;Fang M;Zhong YX;Tang XL;Zhang T;Pan HT
子痫前期和妊娠期高血压可导致子代血管功能损害。在我们以前的工作中，我们描述了先兆子痫患者脐动脉组织的蛋白质表达谱。为了深入了解先兆子痫母鼠出生的成年大鼠血管功能障碍的机制，我们通过使用iTRAQ同量异位素标签和2D纳米LC-MS/MS分析了胸主动脉组织。通过使用iTRAQ方法，我们分析了1825种蛋白质，其中106种在胸主动脉中表现出显着不同的表达。免疫途径分析（IPA）显示，大部分差异表达蛋白（DEPs）与心血管功能相关。进一步的分析表明，葡萄糖-6-磷酸脱氢酶（G6 PD），其被miR-423- 5 p抑制并被TP 53激活，对心血管功能具有最强的影响。Western blotting证实，G6 PD在胸主动脉组织中的表达上调。另外两种血管功能相关蛋白，富含半胱氨酸和甘氨酸的蛋白2（CSRP 2）和微管蛋白α-4 A（TUBA 4A）的表达上调，如质谱（MS）所示。虽然这些结果需要进一步的功能验证，但这些数据提供了与先兆子痫母亲的成年后代血管功能受损相关的新发现。在线版本包含补充材料，可通过10.1186/s12014-021-09327-9获得。
Preeclampsia and gestational hypertension can cause vascular function impairment in offspring. In our previous work, we described the protein expression profiles of umbilical artery tissues from patients with preeclampsia. To gain insights into the mechanisms of vascular dysfunction in adult rats born to preeclamptic dams, we analyzed thoracic aorta tissues by using iTRAQ isobaric tags and 2D nano LC-MS/MS. By using the iTRAQ method, we analyzed 1825 proteins, of which 106 showed significantly different expression in the thoracic aortic. Ingenuity pathway analysis (IPA) showed that the majority of differentially expressed proteins (DEPs) were associated with cardiovascular function. Further analysis indicated that glucose-6-phosphate dehydrogenase (G6PD), which is inhibited by miR-423-5p and activated by TP53, had the strongest effect on cardiovascular function. The expression of G6PD was upregulated in thoracic aorta tissues, as confirmed by Western blotting. The expression of two other vascular function-related proteins, cysteine- and glycine-rich protein 2 (CSRP2) and tubulin alpha-4 A (TUBA4A), was upregulated, as demonstrated by mass spectrometry (MS). Although the results require further functional validation, these data provide novel findings related to vascular function impairment in the adult offspring of preeclamptic mothers. The online version contains supplementary material available at 10.1186/s12014-021-09327-9.
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DOI：
10.1042/cs20110627
发表时间：
2012-07
期刊：
Clinical science (London, England : 1979)
影响因子：
--
作者：
Davis EF;Newton L;Lewandowski AJ;Lazdam M;Kelly BA;Kyriakou T;Leeson P
通讯作者：
Leeson P
影响因子：
14.9
作者：
Ma J;Chen T;Wu S;Yang C;Bai M;Shu K;Li K;Zhang G;Jin Z;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y
影响因子：
8
作者：
Ambartsumian, N;Klingelhöfer, J;Lukanidin, E
通讯作者：
Lukanidin, E
影响因子：
9.8
作者：
Coates, Brian J.;Broderick, Tom L.;Standley, Cynthia A.
通讯作者：
Standley, Cynthia A.
影响因子：
6.2
作者：
Gupta, Sajal;Aziz, Nabil;Agarwal, Ashok
通讯作者：
Agarwal, Ashok