Photothermal Response Induced by Nanocage-Coated Artificial Extracellular Matrix Promotes Neural Stem Cell Differentiation.
Photothermal Response Induced by Nanocage-Coated Artificial Extracellular Matrix Promotes Neural Stem Cell Differentiation.
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由纳米涂层人造细胞外基质引起的光热反应促进了神经干细胞的分化。
DOI：
10.3390/nano11051216
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发表时间：
2021-05-04
期刊：
影响因子：
--
通讯作者：
Griffin RJ
中科院分区：
文献类型：
--
作者：
Jung S;Harris N;Niyonshuti II;Jenkins SV;Hayar AM;Watanabe F;Jamshidi-Parsian A;Chen J;Borrelli MJ;Griffin RJ
增加神经干细胞分化为神经元的比例的策略对于神经退行性疾病的治疗至关重要。在体外，已经发现细胞外基质组成和形貌是干细胞分化的重要因素。我们已经开发了一种新的人工细胞外基质（aECM）形成的附加金纳米笼（AuNCs）的玻璃盖玻片。在这些金纳米笼涂覆的表面（AuNC-aECM）上培养大鼠神经干细胞（rNSCs）后，我们观察到44.6%的rNSCs分化为神经元，而在层粘连蛋白涂覆的盖玻片上生长的细胞仅为27.9%。我们将激光照射到AuNC-aECMs上，以在细胞中引入精确量的光热诱导热休克。我们的研究结果表明，激光诱导的热刺激的AuNC-aECMs进一步增强神经元分化（56%），这取决于所使用的激光强度。对这些光热效应的反应增加了rNSCs中热休克蛋白27、70和90α的表达。树突的复杂性分析表明，这种热刺激促进神经元的成熟，增加树突长度的热剂量增加。此外，我们发现，生长在AuNC-aECMs激光照射后的细胞表现出动作电位，并增加了电压门控Na+通道的表达相比，层粘连蛋白包被的玻璃盖玻片。这些结果表明，在AuNC-aECM上生长的细胞中诱导的光热响应可用于产生大量具有改善的电化学性质的功能性神经元，其可潜在地移植到受损的中枢神经系统中以提供替代神经元并恢复失去的功能。
Strategies to increase the proportion of neural stem cells that differentiate into neurons are vital for therapy of neurodegenerative disorders. In vitro, the extracellular matrix composition and topography have been found to be important factors in stem cell differentiation. We have developed a novel artificial extracellular matrix (aECM) formed by attaching gold nanocages (AuNCs) to glass coverslips. After culturing rat neural stem cells (rNSCs) on these gold nanocage-coated surfaces (AuNC-aECMs), we observed that 44.6% of rNSCs differentiated into neurons compared to only 27.9% for cells grown on laminin-coated glass coverslips. We applied laser irradiation to the AuNC-aECMs to introduce precise amounts of photothermally induced heat shock in cells. Our results showed that laser-induced thermal stimulation of AuNC-aECMs further enhanced neuronal differentiation (56%) depending on the laser intensity used. Response to these photothermal effects increased the expression of heat shock protein 27, 70, and 90α in rNSCs. Analysis of dendritic complexity showed that this thermal stimulation promoted neuronal maturation by increasing dendrite length as thermal dose was increased. In addition, we found that cells growing on AuNC-aECMs post laser irradiation exhibited action potentials and increased the expression of voltage-gated Na+ channels compared to laminin-coated glass coverslips. These results indicate that the photothermal response induced in cells growing on AuNC-aECMs can be used to produce large quantities of functional neurons, with improved electrochemical properties, that can potentially be transplanted into a damaged central nervous system to provide replacement neurons and restore lost function.
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影响因子：
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作者：
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DOI：
10.1111/j.1365-2443.2010.01413.x
发表时间：
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期刊：
Genes to cells : devoted to molecular & cellular mechanisms
影响因子：
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作者：
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通讯作者：
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影响因子：
34.7
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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