Investigation of Blood Coagulation Using Impedance Spectroscopy: Toward Innovative Biomarkers to Assess Fibrinogenesis and Clot Retraction.
Investigation of Blood Coagulation Using Impedance Spectroscopy: Toward Innovative Biomarkers to Assess Fibrinogenesis and Clot Retraction.
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DOI：
10.3390/biomedicines10081833
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发表时间：
2022-07-29
期刊：
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究的重点是基于血液阻抗幅度测量新技术的凝血评估。通过再钙化人体血液的阻抗表征，可以识别与纤维蛋白形成（第一个标记）和凝块回缩（第二个标记）相关的两个重要生物标记（即可测量指标）。凝血共聚焦显微镜提供了对凝血过程中发生的所有事件的完整视觉分析，验证了阻抗生物标志物的重要性。通过分析凝血过程中血液的阻抗相位角（Φ），以及回缩后排出的凝块和血清的阻抗相位角（Φ），可以进一步阐明第二个标记的来源。最后，对同一供体的血液样本同时进行阻抗幅度分析和旋转血栓弹力测试（ROTEM®）；结果指出第一个标志物与凝血时间有关。所开发的技术对凝血产生了全面且不断发展的见解，使得实时逐步跟踪整个过程成为可能。此外，这种方法允许在任何材料的表面上测试凝血，为与接触凝血（即人工植入物上发生的血栓形成）相关的新研究奠定了基础。在不久的将来，阻抗谱可用于心血管假体的材料表征，其特性可以使用有效的生物标志物进行原位和/或在线监测。
This study focused on a coagulation assessment based on the novel technique of blood-impedance-magnitude measurement. With the impedance characterization of recalcified human blood, it was possible to identify two significative biomarkers (i.e., measurable indicators) related to fibrin formation (1st marker) and clot retraction (2nd marker). The confocal microscopy of clotting blood provided a complete visual analysis of all the events occurring during coagulation, validating the significance of the impedance biomarkers. By analyzing the impedance phase angle (Φ) of blood during coagulation, as well as those of the clot and serum expelled after retraction, it was possible to further clarify the origin of the 2nd marker. Finally, an impedance-magnitude analysis and a rotational thromboelastometry test (ROTEM®) were simultaneously performed on blood sampled from the same donor; the results pointed out that the 1st marker was related to clotting time. The developed technique gives rise to a comprehensive and evolutive insight into coagulation, making it possible to progressively follow the whole process in real time. Moreover, this approach allows coagulation to be tested on any materials’ surface, laying the ground for new studies related to contact coagulation, meaning, thrombosis occurring on artificial implants. In a near future, impedance spectroscopy could be employed in the material characterization of cardiovascular prostheses whose properties could be monitored in situ and/or online using effective biomarkers.
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影响因子：
7.5
作者：
Dargaud, Y.;Luddington, R.;Negrier, C.
通讯作者：
Negrier, C.
影响因子：
3.7
作者：
通讯作者：
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影响因子：
3.3
作者：
Denessen, E. J. S.;Van den Kerkhof, D. L.;Henskens, Y. M. C.
通讯作者：
Henskens, Y. M. C.
DOI：
10.1186/1757-7241-21-29
发表时间：
2013-04-16
期刊：
Scandinavian journal of trauma, resuscitation and emergency medicine
影响因子：
--
作者：
da Luz LT;Nascimento B;Rizoli S
通讯作者：
Rizoli S
DOI：
10.1002/smll.201600274
发表时间：
2016-08
期刊：
Small (Weinheim an der Bergstrasse, Germany)
影响因子：
--
作者：
Li Z;Li X;McCracken B;Shao Y;Ward K;Fu J
通讯作者：
Fu J