Osteoclast-independent bone resorption by fibroblast-like cells.
Osteoclast-independent bone resorption by fibroblast-like cells.
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DOI：
10.1186/ar752
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发表时间：
2003
影响因子：
4.9
通讯作者：
Aicher WK
中科院分区：
文献类型：
--
作者：
Pap T;Claus A;Ohtsu S;Hummel KM;Schwartz P;Drynda S;Pap G;Machner A;Stein B;George M;Gay RE;Neumann W;Gay S;Aicher WK
到目前为止，间充质细胞仅间接地与骨吸收有关，并假设骨的降解仅与破骨细胞的特定功能有关。在这里，我们显示，在无菌假体松动中，骨表面的侵袭性成纤维细胞积极促进骨吸收，这是独立于破骨细胞的。在两个独立的模型(严重联合免疫缺陷小鼠共植入模型和牙本质凹陷形成试验)中，这些细胞产生类似于早期破骨细胞吸收的骨吸收迹象。在无菌假体松动的动物模型(即脑内自我刺激的大鼠)中，研究表明，这些成纤维细胞在分化早期就获得了降解骨的能力。在刺激下，这种成纤维细胞很容易释放酸性成分，从而降低细胞周围环境的pH值。通过使用特定的抑制剂，细胞周围的酸化被证明涉及液泡型ATPase的作用。尽管成纤维细胞作为间充质来源的细胞被认为不具有吸收骨的能力，但本研究提供了第一个证据来挑战这一广泛持有的信念。研究表明，在病理条件下，成纤维细胞样细胞不仅可以促进骨吸收，而且可以积极地促进骨吸收。因此，这些细胞应该被认为是治疗骨破坏性疾病的新靶点。
To date, mesenchymal cells have only been associated with bone resorption indirectly, and it has been hypothesized that the degradation of bone is associated exclusively with specific functions of osteoclasts. Here we show, in aseptic prosthesis loosening, that aggressive fibroblasts at the bone surface actively contribute to bone resorption and that this is independent of osteoclasts. In two separate models (a severe combined immunodeficient mouse coimplantation model and a dentin pit formation assay), these cells produce signs of bone resorption that are similar to those in early osteoclastic resorption. In an animal model of aseptic prosthesis loosening (i.e. intracranially self-stimulated rats), it is shown that these fibroblasts acquire their ability to degrade bone early on in their differentiation. Upon stimulation, such fibroblasts readily release acidic components that lower the pH of their pericellular milieu. Through the use of specific inhibitors, pericellular acidification is shown to involve the action of vacuolar type ATPases. Although fibroblasts, as mesenchymal derived cells, are thought to be incapable of resorbing bone, the present study provides the first evidence to challenge this widely held belief. It is demonstrated that fibroblast-like cells, under pathological conditions, may not only enhance but also actively contribute to bone resorption. These cells should therefore be considered novel therapeutic targets in the treatment of bone destructive disorders.
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DOI：
10.1152/ajpcell.1995.268.1.c210
发表时间：
1995-01-01
影响因子：
5.5
作者：
MCKINNEY, LC;MORAN, A
通讯作者：
MORAN, A
影响因子：
2.8
作者：
Pap, G;Eberhardt, R;Neumann, W
通讯作者：
Neumann, W
影响因子：
27.4
作者：
Sabokbar, A;Fujikawa, Y;Athanasou, NA
通讯作者：
Athanasou, NA
影响因子：
4
作者：
Grimbacher, B;Aicher, WK;Eibel, H
通讯作者：
Eibel, H
DOI：
10.2106/00004623-198365050-00001
发表时间：
1983-01-01
影响因子：
5.3
作者：
GOLDRING, SR;SCHILLER, AL;HARRIS, WH
通讯作者：
HARRIS, WH