Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium.
Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium.
复制标题
Jagged1介导的Notch信号激活在人角膜缘上皮分化和复层中的作用
作者：
González S;Halabi M;Ju D;Tsai M;Deng SX
Notch信号通路在增殖和分化中起关键作用。我们研究了Jagged 1（Jag 1）介导的Notch信号激活在人角膜缘干/祖细胞（LSC）群体和角膜缘上皮层化中的作用。在Notch信号传导激活后，干/祖细胞群体、上皮分层和增殖标记物表达的量减少。角膜上皮细胞分化程度也有所增加。在Jag 1的存在下，不对称分裂减少，极性蛋白Par 3的表达模式，通常存在于基底细胞的顶侧膜，分散在细胞中。我们提出了一种机制，其中Notch激活Jag 1减少p63表达在基底层，这反过来又减少分层，减少不对称分裂的数量，并增加分化。
The Notch signaling pathway plays a key role in proliferation and differentiation. We investigated the effect of Jagged 1 (Jag1)-mediated Notch signaling activation in the human limbal stem/progenitor cell (LSC) population and the stratification of the limbal epithelium in vitro. After Notch signaling activation, there was a reduction in the amount of the stem/progenitor cell population, epithelial stratification, and expression of proliferation markers. There was also an increase of the corneal epithelial differentiation. In the presence of Jag1, asymmetric divisions were decreased, and the expression pattern of the polarity protein Par3, normally present at the apical-lateral membrane of basal cells, was dispersed in the cells. We propose a mechanism in which Notch activation by Jag1 decreases p63 expression at the basal layer, which in turn reduces stratification by decreasing the number of asymmetric divisions and increases differentiation.
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影响因子：
16.2
作者：
Bultje, Ronald S.;Castaneda-Castellanos, David R.;Jan, Lily Yen;Jan, Yuh-Nung;Kriegstein, Arnold R.;Shi, Song-Hai
通讯作者：
Shi, Song-Hai
DOI：
10.1089/ten.tec.2016.0388
发表时间：
2017-04
期刊：
Tissue engineering. Part C, Methods
影响因子：
--
作者：
González S;Chen L;Deng SX
通讯作者：
Deng SX
影响因子：
64.5
作者：
CHENN, A;MCCONNELL, SK
通讯作者：
MCCONNELL, SK
影响因子：
2.9
作者：
BREITKREUTZ, D;BOHNERT, A;FUSENIG, NE
通讯作者：
FUSENIG, NE
影响因子：
10.5
作者：
Koster, MI;Kim, S;Roop, DR
通讯作者：
Roop, DR