A NEW CLASS OF HUMAN IMMUNOGLOBULINS. I. A UNIQUE MYELOMA PROTEIN.
A NEW CLASS OF HUMAN IMMUNOGLOBULINS. I. A UNIQUE MYELOMA PROTEIN.
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DOI：
10.1084/jem.121.1.171
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发表时间：
1965-01-01
期刊：
影响因子：
--
通讯作者：
FAHEY JL
中科院分区：
文献类型：
--
作者：
ROWE DS;FAHEY JL
从S. J.，一名患有多发性骨髓瘤的患者被分离并表征。它在许多方面与其他骨髓瘤蛋白相似。色葡萄J.myeloma蛋白质在区带电泳上在慢β球蛋白区出现一个明显的峰，在淀粉凝胶电泳上出现一条带，在超电泳中在7.04S处沉淀。木瓜蛋白酶和半胱氨酸处理产生Fc（快）和Fab（慢）片段。骨髓瘤蛋白的还原和烷基化以约3：1的比例产生重链和轻链。色葡萄J.骨髓瘤蛋白具有L型（II型）轻链。这些抗原性类似于在该患者中也发现的Bence Jones蛋白。色葡萄J.骨髓瘤蛋白在其重链的性质方面是独特的。骨髓瘤蛋白（及其重链和Fc片段）不含IgG、伊加或IgM特异性抗原决定簇。然而，骨髓瘤蛋白（及其重链）确实含有抗原决定簇，这是一类新的免疫球蛋白的特征。色葡萄J.骨髓瘤蛋白在其对正常IgG代谢的影响和木瓜蛋白酶消化产生的Fc片段的电泳迁移率方面也是不寻常的。没有证据表明S.蛋白质是恶性细胞代谢的严重异常产物。研究了S.骨髓瘤蛋白（及其重链）被认为在很大程度上代表了在一小部分正常免疫球蛋白群体中发现的特性。
The unique myeloma protein from S. J., a patient with multiple myeloma, was isolated and characterized. It resembled other myeloma proteins in many respects. The S. J. myeloma protein migrated in a distinct peak in the slow β-globulin region on zone electrophoresis, appeared as a single band on starch gel electrophoresis, and sedimented at 7.04S in the ultracentrifuge. Papain and cysteine treatment produced Fc (fast) and Fab (slow) fragments. Reduction and alkylation of the myeloma protein produced heavy and light chains in a ratio of approximately 3:1. The S. J. myeloma protein had type L (type II) light chains. These were antigenically similar to the Bence Jones protein also found in this patient. The S. J. myeloma protein was unique in the properties of its heavy chains. The myeloma protein (and its heavy chains and Fc pieces) did not contain antigenic determinants specific for IgG, IgA, or IgM. The myeloma protein (and its heavy chains), however, did contain antigenic determinants which are characteristic of a new class of immunoglobulin. The S. J. myeloma protein was unusual also in its effect on the metabolism of normal IgG and in the electrophoretic mobility of the Fc fragment produced by papain digestion. No evidence was obtained to indicate that the entire heavy polypeptide of the S. J. protein was a grossly abnormal product of malignant cell metabolism. The unique properties of the S. J. myeloma protein (and its heavy chains) are believed to represent, in large measure, properties to be found in a small part of the normal immunoglobulin population.
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免疫球蛋白与人类中某些相关副蛋白之间的抗原关系。
DOI：
10.1084/jem.114.4.521
发表时间：
1961-09-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Franklin EC;Stanworth DR
通讯作者：
Stanworth DR
DOI：
10.1016/0019-2791(64)90076-x
发表时间：
1964-01-01
期刊：
IMMUNOCHEMISTRY
影响因子：
--
作者：
FAHEY, JL
通讯作者：
FAHEY, JL
DOI：
10.1084/jem.120.2.253
发表时间：
1964-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
GREY HM;KUNKEL HG
通讯作者：
KUNKEL HG
从L和H多肽链的7s分子重构的抗体和γ-球蛋白。
影响因子：
15.3
作者：
OLINS, D E;EDELMAN, G M
通讯作者：
EDELMAN, G M
影响因子：
64.8
作者：
POULIK, MD;EDELMAN, GM
通讯作者：
EDELMAN, GM