Early recovery from cow's milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow's milk epitopes.
Early recovery from cow's milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow's milk epitopes.
复制标题
牛奶过敏的早期恢复与IgE降低并增加IgG4与牛奶表位的结合有关。
DOI：
10.1016/j.jaci.2010.03.025
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发表时间：
2010-06
影响因子：
14.2
通讯作者：
Sampson, Hugh A.
中科院分区：
文献类型：
--
作者：
Savilahti, Emma M.;Rantanen, Ville;Lin, Jing S.;Karinen, Sirkku;Saarinen, Kristiina M.;Goldis, Marina;Makela, Mika J.;Hautaniemi, Sampsa;Savilahti, Erkki;Sampson, Hugh A.
过敏原特异性IgE、IgG 4和伊加结合的动态和平衡可能有助于牛奶过敏耐受性的发展。分析与牛奶蛋白表位结合的抗体可能有助于预测过敏的自然史。研究早期恢复或持续牛奶过敏患者之间随时间推移与牛奶表位结合的IgE、IgG 4和伊加的差异。我们研究了11例8-9岁持续IgE介导的牛奶过敏患者诊断时（平均年龄7个月）、1年后和随访时（平均年龄8.6岁）的血清样本，以及12例3岁时恢复的患者。我们测量了结合IgE，IgG 4和伊加抗体的顺序表位来自五个主要的牛奶蛋白与肽芯片为基础的免疫测定。我们用一种新的图像处理方法和机器学习预测来分析数据。持续牛奶过敏患者的IgE表位结合模式随时间推移保持稳定，而早期恢复的患者的结合率下降。IgE和IgG 4的结合模式重叠。在早期恢复的患者中，IgG 4结合的信号随着时间的推移而增加，而IgE的信号随着时间的推移而减少。IgE和IgG 4与αs1-、αs2-、β-和κ-酪蛋白区域的结合预测结果具有显著的准确性。获得对牛奶的耐受性与IgE的表位结合减少和IgG 4的相应表位结合的同时增加相关。
Dynamics and balance of allergen specific IgE, IgG4 and IgA binding may contribute to the development of tolerance in cow's milk allergy. Profiling of antibody binding to cow's milk protein epitopes may help in predicting natural history of allergy. To investigate differences in IgE, IgG4 and IgA binding to cow's milk epitopes over time between patients with early recovery or with persisting cow's milk allergy. We studied serum samples at the time of diagnosis (mean age 7 months), one year later and at follow-up (mean age 8.6 years) from 11 patients with persisting IgE-mediated cow's milk allergy at age 8-9 years, and 12 patients who recovered by age 3 years. We measured the binding of IgE, IgG4 and IgA antibodies to sequential epitopes derived from five major cow's milk proteins with a peptide microarray-based immunoassay. We analyzed the data with a novel image processing method together with machine learning prediction. IgE epitope binding patterns were stable over time in patients with persisting cow's milk allergy, whereas binding decreased in patients who recovered early. Binding patterns of IgE and IgG4 overlapped. Among patients who recovered early, the signal of IgG4 binding increased while that of IgE decreased over time. IgE and IgG4 binding to a panel of αs1-, αs2-, β-and κ-casein regions predicted outcome with significant accuracy. Attaining tolerance to cow's milk is associated with decreased epitope binding by IgE and a concurrent increase in corresponding epitope binding by IgG4.
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影响因子：
14.2
作者：
Shreffler, WG;Lencer, DA;Sampson, HA
通讯作者：
Sampson, HA
影响因子：
14.2
作者：
Francis, James N.;James, Louisa K.;Till, Stephen J.
通讯作者：
Till, Stephen J.
影响因子：
5.1
作者：
Vanto, T;Helppilä, S;Koskinen, P
通讯作者：
Koskinen, P
影响因子：
14.2
作者：
Skripak, Justin M.;Matsui, Elizabeth C.;Wood, Robert A.
通讯作者：
Wood, Robert A.
影响因子：
46.9
作者：
Kingsford, Carl;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.