Enhanced bioactivity and osteoinductivity of carboxymethyl chitosan/nanohydroxyapatite/graphene oxide nanocomposites.
Enhanced bioactivity and osteoinductivity of carboxymethyl chitosan/nanohydroxyapatite/graphene oxide nanocomposites.
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羧甲基壳聚糖/纳米羟基磷灰石/氧化石墨烯纳米复合材料增强的生物活性和骨诱导性
DOI：
10.1039/c8ra00383a
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发表时间：
2018-05-14
期刊：
影响因子：
3.9
通讯作者：
Fan, Xianqun
中科院分区：
文献类型：
--
作者：
Yu, Zhang;Xiao, Caiwen;Huang, Yazhuo;Chen, Mingjiao;Wei, Wei;Yang, Xiaoxuan;Zhou, Huifang;Bi, Xiaoping;Lu, Linna;Ruan, Jing;Fan, Xianqun
组织工程方法将生物支架与干细胞结合起来，提供生物替代品，可以修复骨缺损并最终改善组织功能。未来的生物支架应具有良好的骨诱导性。表面化学修饰和粗糙度修饰因其对促进成骨分化的积极作用而被认为是开发生物支架的有价值的策略。然而，目前对这两种策略的协同组合研究甚少。在这项工作中，通过掺杂纳米羟基磷灰石（nHA），化学交联氧化石墨烯（GO）和羧甲基壳聚糖（CMC），制备了具有表面化学（含氧基团）和粗糙度（Rq = 74.1 nm）修饰的纳米工程支架。体外和体内评价纳米工程CMC/nHA/GO支架的生物相容性和成骨诱导能力，并初步探讨纳米工程支架的成骨分化机制。我们的数据表明，CMC/nHA/GO增强的成骨性可能得益于其表面化学和粗糙度，这有利于β1整合素与细胞外基质的相互作用，并激活FAK-ERK信号通路，上调成骨特殊蛋白的表达。该研究表明，经过表面化学和粗糙度修饰的纳米复合支架可以作为一种新的、有前途的组织工程骨替代品。
Tissue engineering approaches combine a bioscaffold with stem cells to provide biological substitutes that can repair bone defects and eventually improve tissue functions. The prospective bioscaffold should have good osteoinductivity. Surface chemical and roughness modifications are regarded as valuable strategies for developing bioscaffolds because of their positive effects on enhancing osteogenic differentiation. However, the synergistic combination of the two strategies is currently poorly studied. In this work, a nanoengineered scaffold with surface chemistry (oxygen-containing groups) and roughness (Rq = 74.1 nm) modifications was fabricated by doping nanohydroxyapatite (nHA), chemically crosslinked graphene oxide (GO) and carboxymethyl chitosan (CMC). The biocompatibility and osteoinductivity of the nanoengineered CMC/nHA/GO scaffold was evaluated in vitro and in vivo, and the osteogenic differentiation mechanism of the nanoengineered scaffold was preliminarily investigated. Our data demonstrated that the enhanced osteoinductivity of CMC/nHA/GO may profit from the surface chemistry and roughness, which benefit the β1 integrin interactions with the extracellular matrix and activate the FAK–ERK signaling pathway to upregulate the expression of osteogenic special proteins. This study indicates that the nanocomposite scaffold with surface chemistry and roughness modifications could serve as a novel and promising bone substitute for tissue engineering.
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