The complete sequence of the smallest known nuclear genome from the microsporidian Encephalitozoon intestinalis.
The complete sequence of the smallest known nuclear genome from the microsporidian Encephalitozoon intestinalis.
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来自微孢子虫脑肠道的最小已知核基因组的完整序列。
DOI：
10.1038/ncomms1082
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发表时间：
2010-09-21
影响因子：
16.6
通讯作者：
Keeling, Patrick J.
中科院分区：
文献类型：
--
作者：
Corradi, Nicolas;Pombert, Jean-Francois;Farinelli, Laurent;Didier, Elizabeth S.;Keeling, Patrick J.
楔形微孢子虫脑孢子虫的基因组被广泛认为是极端压缩和压缩的模式。基因组只有2.9Mbp，编码了大约2,000个密集排列的基因，其他几乎没有。然而，它的姊妹种肠脑孢子虫的核基因组甚至减少了更多；2.3MBP，比已经严重压缩的基因组减少了20%，这引发了一个问题，还能失去什么？在本文中，我们描述了肠杆菌基因组的全序列，并将其与楔形肠杆菌的基因组进行了比较。这两个物种的大部分基因组都有保守的基因含量、顺序和密度。例外的是亚端粒区域，在那里肠杆菌的染色体缺少在楔形肠杆菌中发现的大的序列基因块。在剩余的基因密集的染色体“核心”中，微小的基因间序列和内含子实际上比基因本身更加保守，这表明它们已经达到了完全功能基因组的缩减极限。相关基因组的比较提供了关于它们如何进化的有价值的信息。在这里，描述了已知的最小的肠微孢子虫核基因组的完整序列，并与其较大的姊妹种楔形微孢子虫进行了比较，揭示了即使是最精简的基因组中也是必不可少的部分。
The genome of the microsporidia Encephalitozoon cuniculi is widely recognized as a model for extreme reduction and compaction. At only 2.9 Mbp, the genome encodes approximately 2,000 densely packed genes and little else. However, the nuclear genome of its sister, Encephalitozoon intestinalis, is even more reduced; at 2.3 Mbp, it represents a 20% reduction from an already severely compacted genome, raising the question, what else can be lost? In this paper, we describe the complete sequence of the E. intestinalis genome and its comparison with that of E. cuniculi. The two species share a conserved gene content, order and density over most of their genomes. The exceptions are the subtelomeric regions, where E. intestinalis chromosomes are missing large gene blocks of sequence found in E. cuniculi. In the remaining gene-dense chromosome 'cores', the diminutive intergenic sequences and introns are actually more highly conserved than the genes themselves, suggesting that they have reached the limits of reduction for a fully functional genome. A comparison of related genomes provides valuable information about how they evolve. Here, the complete sequence of the smallest known nuclear genome from the microsporidia E. intestinalis is described and compared with its larger sister E. cuniculi, revealing what parts are indispensable in even the most reduced genomes.
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影响因子：
9.8
作者：
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作者：
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通讯作者：
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通讯作者：
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