3D printed microfluidic device for automated, pressure-driven, valve-injected microchip electrophoresis of preterm birth biomarkers.
3D printed microfluidic device for automated, pressure-driven, valve-injected microchip electrophoresis of preterm birth biomarkers.
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DOI：
10.1007/s00604-022-05303-8
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发表时间：
2022-04-28
影响因子：
5.7
通讯作者：
Woolley, Adam T.
中科院分区：
文献类型：
--
作者：
Esene, Joule E.;Boaks, Mawla;Bickham, Anna, V;Nordin, Gregory P.;Woolley, Adam T.
我们开发了一种3D打印的自动化压力驱动注射微流体系统，用于早产（PTB）相关肽和蛋白质的微芯片电泳（µCE）。形成功能性微阀，膜厚度为5 µm，层暴露时间为450 ms，或膜厚度为10 µm，层暴露时间为300-350 ms。这些阀允许在样品注射期间控制装置微通道中的流体流动，以进行µCE分离。我们使用荧光标记的氨基酸优化了器件设计和µCE条件。样品注入时间为0.5 s，分离电压为450 V（460 V/cm），分离效率和分辨率最好。我们使用荧光标记的PTB生物标志物和532 nm激光激发，在3D打印的微流体装置中展示了压力驱动注射的第一个µCE分离。对于1.5 s的注射时间，两种PTB生物标志物肽1和肽2的检测限分别为400 pM和15 nM，肽2的线性检测范围为50-400 nM。这种3D打印的微流体系统有望在未来将片上样品制备过程与µCE集成，为PTB风险评估提供有希望的可能性。带有集成气动阀的3D打印微流体设备改善了与早产风险相关的微芯片电泳分离生物标志物。
We developed a 3D printed, automated, pressure-driven injection microfluidic system for microchip electrophoresis (µCE) of preterm birth (PTB)-related peptides and proteins. Functional microvalves were formed, either with a membrane thickness of 5 µm and a layer exposure time of 450 ms, or with a membrane thickness of 10 µm and layer exposure times of 300–350 ms. These valves allowed for control of fluid flow in device microchannels during sample injection for µCE separation. We optimized device design and µCE conditions using fluorescently labeled amino acids. A sample injection time of 0.5 s and a separation voltage of 450 V (460 V/cm) yielded the best separation efficiency and resolution. We demonstrated the first µCE separation with pressure-driven injection in a 3D printed microfluidic device using fluorescently labeled PTB biomarkers and 532 nm laser excitation. Detection limits for two PTB biomarkers, peptide 1 and peptide 2, for an injection time of 1.5 s were 400 pM and 15 nM, respectively, and the linear detection range for peptide 2 was 50–400 nM. This 3D printed microfluidic system holds promise for future integration of on-chip sample preparation processes with µCE, offering promising possibilities for PTB risk assessment. 3D printed microfluidic devices with integrated pneumatic valves improve microchip electrophoresis separation biomarkers related to preterm birth risk.
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影响因子：
9.8
作者：
Esplin MS;Merrell K;Goldenberg R;Lai Y;Iams JD;Mercer B;Spong CY;Miodovnik M;Simhan HN;van Dorsten P;Dombrowski M;Eunice Kennedy Shriver National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
通讯作者：
Eunice Kennedy Shriver National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
DOI：
10.1039/d1ay01569a
发表时间：
2021-11-04
期刊：
Analytical methods : advancing methods and applications
影响因子：
--
作者：
Castiaux AD;Selemani MA;Ward MA;Martin RS
通讯作者：
Martin RS
影响因子：
168.9
作者：
Liu, Li;Oza, Shefali;Hogan, Dan;Chu, Yue;Perin, Jamie;Zhu, Jun;Lawn, Joy E.;Cousens, Simon;Mathers, Colin;Black, Robert E.
通讯作者：
Black, Robert E.
影响因子：
7.4
作者：
Beauchamp, Michael J.;Nielsen, Anna V.;Woolley, Adam T.
通讯作者：
Woolley, Adam T.
影响因子：
6.1
作者：
Gong, Hua;Woolley, Adam T.;Nordin, Gregory P.
通讯作者：
Nordin, Gregory P.