Molecular Expression of the Scribble Complex Genes, Dlg, Scrib and Lgl, in Silkworm, Bombyx mori.
Molecular Expression of the Scribble Complex Genes, Dlg, Scrib and Lgl, in Silkworm, Bombyx mori.
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家蚕 Scribble 复合基因 Dlg、Scrib 和 Lgl 的分子表达
DOI：
10.3390/genes4020264
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发表时间：
2013-05-30
期刊：
影响因子：
3.5
通讯作者：
Wei ZJ
中科院分区：
文献类型：
--
作者：
Qi HS;Liu SM;Li S;Wei ZJ
Scribble蛋白复合体基因由致死性巨幼虫（Lgl）、大圆盘（Dlg）和scribble（Scribb）基因组成，是连接人类和果蝇细胞中细胞极性的进化上保守的遗传途径的组分。采用qRT-RCR方法记录Scribble蛋白复合物基因的组织表达和发育变化。在脂肪体、中肠、精巢/卵巢、翅盘、气管、马氏管、血淋巴、前胸腺和丝腺等组织中均检测到Lgl和Scrib基因的表达。Dlg基因主要在睾丸/卵巢中表达，在前胸腺和血淋巴中不表达。在脂肪体中，这三个基因有两个较高的表达阶段。Lgl和Scrib基因在5龄1日龄时表达量最高，而Dlg基因在5龄3日龄时表达量最高。上述结果表明，Scribble复合体基因参与了家蚕蜕皮和翅盘发育的过程。这将有助于进一步阐明Scrib、Dlg和Lgl三个基因的详细生物学功能。森。
The Scribble protein complex genes, consisting of lethal giant larvae (Lgl), discs large (Dlg) and scribble (Scrib) genes, are components of an evolutionarily conserved genetic pathway that links the cell polarity in cells of humans and Drosophila. The tissue expression and developmental changes of the Scribble protein complex genes were documented using qRT-RCR method. The Lgl and Scrib genes could be detected in all the experimental tissues, including fat body, midgut, testis/ovary, wingdisc, trachea, malpighian tubule, hemolymph, prothoracic gland and silk gland. The Dlg gene, mainly expressed only in testis/ovary, could not be detected in prothoracic gland and hemolymph. In fat body, there were two higher expression stages of the three genes. The highest peak of the expression of the Lgl and Scrib genes in wingdisc lay at the 1st day of the 5th instar, but the Dlg gene was at 3rd day of 5th instar. The above results indicate that Scribble complex genes are involved in the process of molting and development of the wingdisc in the silkworm. This will be useful in the future for the elucidation of the detailed biological function of the three genes Scrib, Dlg and Lgl in B. mori.
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影响因子：
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