Elevated systemic antibodies towards commensal gut microbiota in autoinflammatory condition.
Elevated systemic antibodies towards commensal gut microbiota in autoinflammatory condition.
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DOI：
10.1371/journal.pone.0003172
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发表时间：
2008-09-09
期刊：
影响因子：
3.7
通讯作者：
Aminov RI
中科院分区：
文献类型：
--
作者：
Manukyan GP;Ghazaryan KA;Ktsoyan ZA;Khachatryan ZA;Arakelova KA;Kelly D;Grant G;Aminov RI
家族性地中海热（FMF）是一种自身炎症性疾病，其特征是急性、自限性发热和浆膜炎发作以及缓解期慢性亚临床炎症。在这里，我们研究了这种情况对针对常见肠道细菌的全身抗体水平的影响。通过ELISA测定了FMF患者在疾病的各个阶段和健康对照中对拟杆菌属、副拟杆菌属、埃希氏菌属、肠球菌属和乳酸杆菌属抗原的全身抗体水平。缓解期与发作期之间差异无统计学意义。与对照组相比，FMF中针对拟杆菌属、副拟杆菌属、埃希氏菌属和肠球菌属抗原的IgG抗体显著增加，而伊加水平没有受到显著影响。Western blot分析表明，FMF中的IgG对多种细菌抗原具有反应性。进行血清学表达克隆以鉴定这些抗原。没有发现单一的优势抗原;反应是普遍的，针对拟杆菌属、副拟杆菌属、埃希氏菌属和其他肠道微生物的各种蛋白质。这种自身炎性综合征的特征在于对肠道微生物群的全身反应性增加。这可能是FMF中炎性小体的超敏反应的结果，其触发炎症并有助于细菌和细菌抗原通过肠道屏障的过度易位。
Familial Mediterranean fever (FMF) is an autoinflammatory condition, which is characterized by acute, self-limiting episodes of fever and serositis and chronic subclinical inflammation in remission. Here we investigated the consequence of this condition on the level of systemic antibodies directed towards common intestinal bacteria. The level of systemic antibodies towards the antigens of Bacteroides, Parabacteroides, Escherichia, Enteroccocus and Lactobaccilus was measured by ELISA in FMF patients at various stages of the disease and in healthy controls. The difference between remission and attack was not significant. IgG antibodies against the antigens of Bacteroides, Parabacteroides, Escherichia and Enteroccocus were significantly increased in FMF compared to control while IgA levels were not significantly affected. Western blot analyses demonstrated the IgG reactivity against multiple antigens of commensal bacteria in FMF. Serological expression cloning was performed to identify these antigens. No single dominant antigen was identified; the response was generalized and directed against a variety of proteins from Bacteroides, Parabacteroides, Escherichia, and other gut commensals. This autoinflammatory syndrome is characterized by the increased systemic reactivity against commensal gut microbiota. This is probably the consequence of hypersensitivity of the inflammasome in FMF that triggers the inflammation and contributes to the excessive translocation of bacteria and bacterial antigens through the gut barrier.
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影响因子：
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