OsGIF1 Positively Regulates the Sizes of Stems, Leaves, and Grains in Rice.
OsGIF1 Positively Regulates the Sizes of Stems, Leaves, and Grains in Rice.
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OsGIF1 正向调节水稻茎、叶和籽粒的大小
DOI：
10.3389/fpls.2017.01730
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发表时间：
2017
影响因子：
5.6
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
He Z;Zeng J;Ren Y;Chen D;Li W;Gao F;Cao Y;Luo T;Yuan G;Wu X;Liang Y;Deng Q;Wang S;Zheng A;Zhu J;Liu H;Wang L;Li P;Li S
生长调节因子 (GRF) 相互作用因子 (GIF) 参与拟南芥的多个发育过程。我们之前表明，OsGIF1 表达的上调可改善水稻籽粒尺寸。然而，OsGIF1 是否参与其他发育过程仍不清楚。在这里，我们报告了 OsGIF1 对水稻器官大小调节的多效性作用。通过 CRISPR/Cas9 策略的过度表达和功能性敲除表明，OsGIF1 不仅能正向调节水稻叶、茎和籽粒的大小，还能影响水稻的繁殖。基于 qRT-PCR 和 GUS（β-葡萄糖醛酸酶）组织化学染色的表达谱表明，OsGIF1 在各种水稻组织中存在差异表达，这与其在调节多个水稻器官发育中的作用一致。此外，我们发现 OsGIF1-GFP 优先定位于细胞核，这支持了其作为转录辅助因子的作用。进一步的组织学分析表明，OsGIF1 可能通过调节细胞大小来影响水稻器官的大小。我们的结果表明 OsGIF1 在营养和生殖发育过程中发挥重要作用，对水稻育种具有重要意义。
Growth-regulating factor (GRF) interacting factors (GIFs) are involved in several developmental processes in Arabidopsis. We previously showed that upregulation of OsGIF1 expression improves rice grain size. However, whether OsGIF1 is involved in other developmental processes remains unclear. Here, we report pleiotropic effects of OsGIF1 on rice organ size regulation. Overexpression and functional knock-out via a CRISPR/Cas9 strategy revealed that OsGIF1 not only positively regulates the sizes of rice leaf, stem, and grain but also influences rice reproduction. Expression profiles based on both qRT-PCR and GUS (β-glucuronidase) histochemical staining suggested that OsGIF1 is differentially expressed across various rice tissues, consistent with its roles in regulating the development of multiple rice organs. Additionally, we found that OsGIF1-GFP localized preferentially in the nucleus, which supports its proposed role as a transcriptional cofactor. Further histological analysis suggested that OsGIF1 affected rice organ size possibly by regulating cell size. Our results suggest that OsGIF1 plays important roles in vegetative and reproductive developmental processes, with important implications for rice breeding.
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