Arabidopsis CROWDED NUCLEI (CRWN) proteins are required for nuclear size control and heterochromatin organization.
Arabidopsis CROWDED NUCLEI (CRWN) proteins are required for nuclear size control and heterochromatin organization.
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DOI：
10.1186/1471-2229-13-200
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发表时间：
2013-12-05
影响因子：
5.3
通讯作者：
Richards EJ
中科院分区：
文献类型：
--
作者：
Wang H;Dittmer TA;Richards EJ
植物的核表面上类似于动物和真菌的核，但在这些真核生物谱系中构成核组织的机制和过程似乎在进化上是截然不同的。在植物的核结构元件中候选的是NMCP(核基质组成蛋白)家族的卷曲卷曲蛋白。利用遗传学和细胞学的方法，我们分析了拟南芥中由crwn基因编码的四个NMCP家族蛋白的功能，它们最初被命名为LINC(小核)。Crwn蛋白对于生存是必不可少的，这一点从无法恢复在所有四个crwn基因中都有中断的突变中得到了证明。不同组合缺失的突变体表现出核组织的改变，包括核大小缩小、核形状异常和结构异染色质的空间组织异常。我们的结果表明CRWN同源基因的功能多样化；CRWN1在控制核的大小和形状方面起主导作用，其次是CRWN4。适当的染色中心组织对CRWN4的缺失最为敏感。在内复制水平没有相应降低的情况下，CRWN突变体的核体积减少会导致平均核DNA密度的增加。我们的发现表明，CRWN蛋白是植物细胞核的重要结构成分，在异染色质组织和核形态控制中发挥着不同的作用。
Plant nuclei superficially resemble animal and fungal nuclei, but the machinery and processes that underlie nuclear organization in these eukaryotic lineages appear to be evolutionarily distinct. Among the candidates for nuclear architectural elements in plants are coiled-coil proteins in the NMCP (Nuclear Matrix Constituent Protein) family. Using genetic and cytological approaches, we dissect the function of the four NMCP family proteins in Arabidopsis encoded by the CRWN genes, which were originally named LINC (LITTLE NUCLEI). CRWN proteins are essential for viability as evidenced by the inability to recover mutants that have disruptions in all four CRWN genes. Mutants deficient in different combinations of the four CRWN paralogs exhibit altered nuclear organization, including reduced nuclear size, aberrant nuclear shape and abnormal spatial organization of constitutive heterochromatin. Our results demonstrate functional diversification among CRWN paralogs; CRWN1 plays the predominant role in control of nuclear size and shape followed by CRWN4. Proper chromocenter organization is most sensitive to the deficiency of CRWN4. The reduction in nuclear volume in crwn mutants in the absence of a commensurate reduction in endoreduplication levels leads to an increase in average nuclear DNA density. Our findings indicate that CRWN proteins are important architectural components of plant nuclei that play diverse roles in both heterochromatin organization and the control of nuclear morphology.
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影响因子：
6.9
作者：
Ciska M;Masuda K;Moreno Díaz de la Espina S
通讯作者：
Moreno Díaz de la Espina S
影响因子：
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作者：
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Schubert, I
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3.3
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