Oral immunization with Lactococcus lactis secreting attenuated recombinant staphylococcal enterotoxin B induces a protective immune response in a murine model.
Oral immunization with Lactococcus lactis secreting attenuated recombinant staphylococcal enterotoxin B induces a protective immune response in a murine model.
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分泌减毒重组葡萄球菌肠毒素 B 的乳酸乳球菌口服免疫可在小鼠模型中诱导保护性免疫反应。
DOI：
10.1186/1475-2859-12-32
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发表时间：
2013-04-05
影响因子：
6.4
通讯作者：
Paschoalin VM
中科院分区：
文献类型：
--
作者：
Asensi GF;de Sales NF;Dutra FF;Feijó DF;Bozza MT;Ulrich RG;Miyoshi A;de Morais K;Azevedo VA;Silva JT;Le Loir Y;Paschoalin VM
金黄色葡萄球菌不限于在人类和维持热稳态的动物物种中发现。加工设备清洁不充分或处理不当会污染加工食品，导致严重食物中毒。葡萄球菌肠毒素B（SE B）是一种强有力的超抗原外毒素，约50%的临床分离株产生SE B。金黄色葡萄球菌，并与大量食物中毒和诱导中毒性休克综合征有关。成功克隆了编码重组SEB（rSEB）的基因序列，该基因序列不具有超抗原活性，并在食品级乳酸菌乳酸乳球菌中以细胞质或分泌形式表达。在细胞质或培养基中检测到的重组蛋白表现出预期的分子量，并被SEB-多克隆抗体识别。用重组L.乳酸链球菌菌株在S.金黄色葡萄球菌感染。免疫小鼠在腹腔内感染沙门氏菌后存活。金黄色葡萄球菌SEB生产菌株。计数S。rSEB免疫小鼠脾脏中金黄色葡萄球菌的表达显著降低。rSEB免疫的小鼠在粪便和血清中分别显示出显著的抗SEB伊加和IgG滴度。重组L.乳酸菌菌株能够在小鼠中引发细胞或全身免疫应答，如果rSEB以其细胞质或分泌形式产生，则没有显著差异。而重组L.表达胞质rSEB的乳酸菌使受攻击小鼠的存活率增加了43%。这些结果表明了L.在小鼠模型中，在致死性S.金黄色葡萄球菌攻击。
Staphylococcus aureus is unrestrictedly found in humans and in animal species that maintain thermal homeostasis. Inadequate cleaning of processing equipment or inappropriate handling can contaminate processed food and cause severe food poisoning. Staphylococcal enterotoxin B (SEB), a potent superantigenic exotoxin, is produced by 50% of clinical isolates of S. aureus and is associated with massive food poisoning and with the induction of toxic shock syndrome. A gene sequence encoding a recombinant SEB (rSEB), devoid of superantigenic activity, was successfully cloned and expressed in a cytoplasmic or a secreted form in the food-grade lactic acid bacterium Lactococcus lactis. The recombinant protein detected in the cytoplasm or in the culture medium exhibited the expected molecular mass and was recognized by a SEB-polyclonal antibody. Oral immunization with the recombinant L. lactis strains induced a protective immune response in a murine model of S. aureus infection. Immunized mice survived intraperitoneal challenge with an S. aureus SEB-producer strain. Counts of S. aureus in the spleen of rSEB-immunized mice were significantly reduced. The rSEB-immunized mice showed significant titers of anti-SEB IgA and IgG in stools and serum, respectively. Both recombinant L. lactis strains were able to elicit cellular or systemic immune responses in mice, with no significant difference if rSEB was produced in its cytoplasmic or secreted form. However, recombinant L. lactis expressing the cytoplasmic rSEB increased the survival rate of the challenged mice by 43%. These findings show the vaccine efficacy of L. lactis carrying an attenuated SEB, in a murine model, following lethal S. aureus challenge.
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影响因子：
5.5
作者：
Chang, Byoung Sun;Moon, Jin San;Park, Yong Ho
通讯作者：
Park, Yong Ho
影响因子：
3.4
作者：
Pocsfalvi, Gabriella;Cacace, Giuseppina;Malorni, Antonio
通讯作者：
Malorni, Antonio
影响因子：
5.5
作者：
Reveneau, N;Geoffroy, MC;Mercenier, A
通讯作者：
Mercenier, A
影响因子：
2.6
作者：
Medina, Marcela;Villena, Julio;Alvarez, Susana
通讯作者：
Alvarez, Susana
影响因子：
5.4
作者：
Pouwels, PH;Leer, RJ;Conway, PL
通讯作者：
Conway, PL