Photoinitiated destruction of composite porphyrin-protein polymersomes.
Photoinitiated destruction of composite porphyrin-protein polymersomes.
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DOI：
10.1021/ja808586q
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发表时间：
2009-03-25
影响因子：
15
通讯作者：
Dmochowski IJ
中科院分区：
文献类型：
--
作者：
Robbins GP;Jimbo M;Swift J;Therien MJ;Hammer DA;Dmochowski IJ
当负载中等浓度（≥1.5 mg/mL）的马脾铁蛋白（HSF）或其无铁变体（HSAF）时，由两亲性二嵌段共聚物（聚合物囊泡）组装的双层囊泡采用不对称结构。铁蛋白和锌卟啉二聚体（PZn 2）的掺入产生光响应性囊泡：用近UV至近IR波长的聚焦光照射在分钟时间尺度上诱导聚合物小体变形和破坏。为了研究这种现象，聚合物囊泡负载了染料标记的铁蛋白和PZn 2。共聚焦显微镜鉴定BODIPY-FL标记的铁蛋白在膜，而Cy 3标记的铁蛋白被发现在膜和整个水的核心。荧光恢复后的光漂白（FRAP）实验证实，Cy 3和BODIPY-FL标记的铁蛋白和PZn 2表现出缓慢的扩散在膜上，与膜协会。此外，微量吸液管抽吸实验表明，增加弹性模量和改变弯曲刚度的囊泡纳入HSAF。最后，一个小分子（生物胞素）被包裹在铁蛋白− PZn 2囊泡中，并在暴露于光下时释放出来。这些数据表明铁蛋白和PZn 2之间的协同作用，铁蛋白的膜结合降低了变形屏障，PZn 2嵌入膜中，收获光能并产生局部加热，可能导致膜出芽。这似乎是一种普遍的蛋白质-聚合物膜现象，因为用牛血清白蛋白或马骨骼肌肌红蛋白替代铁蛋白导致具有类似不对称形态和光敏性的囊泡。
Bilayer vesicles assembled from amphiphilic diblock copolymers (polymersomes) adopt asymmetric structures when loaded with moderate concentrations (≥1.5 mg/mL) of horse spleen ferritin (HSF) or its iron-free variant (HSAF). Incorporation of both ferritin and a zinc porphyrin dimer (PZn2) generates photoresponsive vesicles: irradiation with focused light of near-UV to near-IR wavelengths induces polymersome deformation and destruction on the minute time scale. To investigate this phenomenon, polymersomes were loaded with dye-labeled ferritin and PZn2. Confocal microscopy identified BODIPY-FL-labeled ferritin at the membrane, whereas Cy3-labeled ferritin was found both at the membrane and throughout the aqueous core. Fluorescence recovery after photobleaching (FRAP) experiments confirmed that Cy3- and BODIPY-FL-labeled ferritin and PZn2exhibited slow diffusion at the membrane, consistent with membrane association. Furthermore, micropipette aspiration experiments revealed increased elastic moduli and altered bending rigidity in vesicles incorporating HSAF. Finally, a small molecule (biocytin) was encapsulated within the ferritin−PZn2vesicles and released upon exposure to light. These data indicate synergy between ferritin, whose membrane association lowers the barrier to deformation, and PZn2, which embeds in the membrane, harvests light energy and produces local heating that may lead to membrane budding. This appears to be a general protein−polymer membrane phenomenon, as replacement of ferritin with bovine serum albumin or equine skeletal myoglobin resulted in vesicles with similar asymmetric morphology and photosensitivity.
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影响因子：
15
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
Paasonen, Lauri;Laaksonen, Timo;Urth, Arto
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影响因子：
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作者：
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通讯作者：
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通过将膜结合基质蛋白自组装成流体样的萌芽结构的囊泡形成。
影响因子：
7.8
作者：
Shnyrova, Anna V.;Ayllon, Juan;Mikhalyov, Ilya I.;Villar, Enrique;Zimmerberg, Joshua;Frolov, Vadim A.
通讯作者：
Frolov, Vadim A.