Redistribution of the nuclear mitotic apparatus protein (NuMA) during mitosis and nuclear assembly. Properties of purified NuMA protein.
Redistribution of the nuclear mitotic apparatus protein (NuMA) during mitosis and nuclear assembly. Properties of purified NuMA protein.
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有丝分裂和核组装过程中核有丝分裂装置蛋白 (NuMA) 的重新分布。
DOI：
10.1016/0014-4827(86)90478-7
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发表时间：
1986
影响因子：
3.7
通讯作者：
Pettijohn,DE
中科院分区：
文献类型：
--
作者：
Price,CM;Pettijohn,DE
用抗核有丝分裂器蛋白(NUMA蛋白)的单抗和人自身免疫血清研究了该蛋白的结构及其在细胞内的分布。用免疫亲和层析柱纯化了NUMA蛋白。对这种大的(250kD)核蛋白的研究表明，它是一种高度不对称的磷蛋白。它存在于所有被检查的哺乳动物细胞和一些非哺乳动物的细胞中。对固定细胞的免疫荧光研究表明，在细胞周期的所有阶段，其在所有物种中的细胞内分布基本上相同。免疫印迹分析表明，NUMA蛋白的大小在不同物种中略有不同。在有丝分裂开始时，NUMA蛋白从细胞核重新分布到两个中心体结构，这两个中心体结构后来成为有丝分裂纺锤体极的一部分。这发生在核破裂时，并最终导致NUMA蛋白在有丝分裂纺锤体的极区积累。在后期，蛋白质从纺锤体极区重新分布到重整核中，最初集中在核层和核周蛋白的组装部位。对显微注射荧光抗NUMA抗体的活细胞进行研究，以检测影响NUMA蛋白在体内重新分布的参数。这些实验表明，微管组装对于NUMA蛋白在极区积累是必不可少的。
Monoclonal antibodies and human autoimmune sera specific for the nuclear mitotic apparatus protein (NuMA protein) were applied to study the structure of this protein and its intracellular distribution. The NuMA protein was purified using immuno-affinity columns. Studies on this large (250 kD) nuclear protein indicated that it is a highly asymmetric phosphoprotein. It is present in all mammalian cells examined and in those of some non-mammals. Immunofluorescence studies on fixed cells demonstrated that its intracellular distribution is essentially the same in all species at all stages of the cell cycle. Immunoblot (western blot) analysis showed that the size of the NuMA protein varies slightly in different species. At the onset of mitosis the NuMA protein redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. This occurs at the time of nuclear breakdown and eventually leads to an accumulation of the NuMA protein at the polar region of the mitotic spindle. After anaphase the protein redistributes from the spindle polar region into the reforming nucleus and concentrates initially at the site where nuclear lamins and perichomatin have been reported to assemble. Living cells microinjected with fluorescent anti-NuMA antibodies were studied to examine parameters that effect the redistribution of the NuMA protein in vivo. These experiments indicate that microtubule assembly is essential for the NuMA protein to accumulate in the polar region.
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影响因子：
64.5
作者：
STACEY, DW;ALLFREY, VG
通讯作者：
ALLFREY, VG
影响因子：
64.5
作者：
LYDERSEN, BK;PETTIJOHN, DE
通讯作者：
PETTIJOHN, DE
影响因子：
64.8
作者：
IZANT, JG;WEATHERBEE, JA;MCINTOSH, JR
通讯作者：
MCINTOSH, JR
早期 SV40 DNA 片段和 T 抗原的显微注射。
影响因子：
--
作者：
A. Graessmann;M. Graessmann;C. Mueller
通讯作者：
C. Mueller
哺乳动物细胞有丝分裂因子：初步表征。
DOI：
--
发表时间：
1979
期刊：
Journal of Supramolecular Structure
影响因子：
--
作者：
Prasad S. Sunkara;David A. Wright;Potu N. Rao
通讯作者：
Potu N. Rao