Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata.
Genome-wide identification and expression profiling of invertase gene family for abiotic stresses tolerance in Poncirus trifoliata.
复制标题
枳实非生物胁迫耐受性转化酶基因家族的全基因组鉴定和表达谱分析
DOI：
10.1186/s12870-021-03337-3
复制
发表时间：
2021-11-25
影响因子：
5.3
通讯作者：
Liu JH
中科院分区：
文献类型：
--
作者：
Dahro B;Wang Y;Alhag A;Li C;Guo D;Liu JH
背景
蔗糖(Suc)的降解直接关系到植物对多种非生物胁迫的耐受性。蔗糖酶(INV)不可逆地催化蔗糖降解生成葡萄糖(GLC)和果糖(FRC)。然而，柑橘INV基因家族的个体成员在全基因组范围内的鉴定和功能对非生物胁迫的响应还不完全清楚。
结果
在本报告中，我们在三叶侧耳中鉴定了14个非冗余的PtrINV家族成员，包括7个碱性/中性INV基因(PtrA/NINV1-7)、2个液泡型INV基因(PtrVINV1-2)和5个细胞壁INV亚型(PtrCWINV1-5)。基于生化特征、染色体定位、外显子-内含子结构和进化关系的综合分析，证实了PtrINV的保守性和差异性。此外，对INV基因在几种非生物胁迫过程中在不同组织中的表达分析表明，A/NINV7在INV家族成员对非生物胁迫的应答中起着中心作用。此外，我们的研究结果还表明，耐冷性较强的白三叶、宜昌和中华三种柑橘中Suc、GLC、FRC和总糖含量的高积累与可溶性INV酶活性的提高直接相关，从而证明了可溶性INV酶在柑橘耐冷性中的潜在作用。
结论
这项工作为理解INV基因的生理作用提供了一个框架，并为未来这些基因响应非生物胁迫的功能研究奠定了基础。
Background
Sucrose (Suc) hydrolysis is directly associated with plants tolerance to multiple abiotic stresses. Invertase (INV) enzymes irreversibly catalyze Suc degradation to produce glucose (Glc) and fructose (Frc). However, genome-wide identification and function of individual members of the INV gene family in Poncirus trifoliata or its Citrus relatives in response to abiotic stresses are not fully understood.
Results
In this report, fourteen non-redundant PtrINV family members were identified in P. trifoliata including seven alkaline/neutral INV genes (PtrA/NINV1–7), two vacuolar INV genes (PtrVINV1–2), and five cell wall INV isoforms (PtrCWINV1–5). A comprehensive analysis based on the biochemical characteristics, the chromosomal location, the exon–intron structures and the evolutionary relationships demonstrated the conservation and the divergence of PtrINVs. In addition, expression analysis of INV genes during several abiotic stresses in various tissues indicated the central role of A/NINV7 among INV family members in response to abiotic stresses. Furthermore, our data demonstrated that high accumulation of Suc, Glc, Frc and total sugar contents were directly correlated with the elevated activities of soluble INV enzymes in the cold-tolerant P. trifoliata, C. ichangensis and C. sinensis, demonstrating the potential role of soluble INV enzymes for the cold tolerance of Citrus.
Conclusions
This work offered a framework for understanding the physiological role of INV genes and laid a foundation for future functional studies of these genes in response to abiotic stresses.
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影响因子：
14.9
作者：
Briesemeister S;Rahnenführer J;Kohlbacher O
通讯作者：
Kohlbacher O
DOI：
10.21273/jashs.120.2.286
发表时间：
1995-03-01
影响因子：
1.9
作者：
CHENG, FSH;ROOSE, ML
通讯作者：
ROOSE, ML
PpVIN2是酸性转化酶基因家族成员，对冷藏温度敏感并影响采后桃果实的蔗糖代谢
影响因子：
4.2
作者：
He, Xingxing;Wei, Yingying;Shao, Xingfeng
通讯作者：
Shao, Xingfeng
影响因子：
5.4
作者：
García, R;Asíns, MJ;Carbonell, EA
通讯作者：
Carbonell, EA
影响因子：
11.6
作者：
Jin, Ye;Ni, Di-An;Ruan, Yong-Ling
通讯作者：
Ruan, Yong-Ling