Correlation of meniscal T2* with multiphoton microscopy, and change of articular cartilage T2 in an ovine model of meniscal repair.
Correlation of meniscal T2* with multiphoton microscopy, and change of articular cartilage T2 in an ovine model of meniscal repair.
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DOI：
10.1016/j.joca.2013.04.020
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发表时间：
2013-08
影响因子：
7
通讯作者：
Potter, H. G.
中科院分区：
文献类型：
--
作者：
Koff, M. F.;Shah, P.;Pownder, S.;Romero, B.;Williams, R.;Gilbert, S.;Maher, S.;Fortier, L. A.;Rodeo, S. A.;Potter, H. G.
使用超短回波时间（UTE）磁共振成像（MRI）将半月板T2 * 弛豫时间与定量显微镜方法相关联，并确定半月板修复对术后软骨T2值的影响。在28只杂交成熟母羊的一肢前角创建并修复了内侧踝关节撕裂。在术后0、4和8个月时，对撕裂和非手术肢体进行MR扫描，以进行形态学评价、骨关节T2 * 值和软骨T2值。采用多光子显微镜（MPM）分析和生物力学测试分析了来自两条肢体的样本。发现修复肢体的T2 * 值比对照肢体显著延长，p <0.0001。修复组和对照组的前角T2 * 均无区域性差异。修复肢体的软骨T2值延长，主要是前部，并且在8个月时的生物力学失效力往往低于非手术肢体。MPM自体荧光和二次谐波产生数据与8个月时的T2 * 值相关（ρ =-0.48，p = 0.06）。T2~*映射对检测植被结构和组成的时间和地带性差异是敏感的。半月板MPM和软骨T2值指示在存在半月板修复的情况下组织完整性的变化。
To correlate meniscal T2* relaxation times using ultra-short echo time (UTE) magnetic resonance imaging (MRI) with quantitative microscopic methods, and to determine the effect of meniscal repair on post-operative cartilage T2 values. A medial meniscal tear was created and repaired in the anterior horn of one limb of 28 crossbred mature ewes. MR scans for morphological evaluation, meniscal T2* values, and cartilage T2 values were acquired at 0, 4 and 8 months post-operatively for the Tear and Non-Op limb. Samples of menisci from both limbs were analyzed using multiphoton microscopy (MPM) analysis and biomechanical testing. Significantly prolonged meniscal T2* values were found in repaired limbs than in control limbs, p<0.0001. No regional differences of T2* were detected for either the repaired or control limbs in the anterior horn. Repaired limbs had prolonged cartilage T2 values, primarily anteriorly, and tended to have lower biomechanical force to failure at 8 months than Non-Op limbs. MPM autofluorescence and second harmonic generation data correlated with T2* values at 8 months (ρ=−0.48, p=0.06). T2* mapping is sensitive to detecting temporal and zonal differences of meniscal structure and composition. Meniscal MPM and cartilage T2 values indicate changes in tissue integrity in the presence of meniscal repair.
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影响因子：
4.8
作者：
Kelly, Bryan T.;Robertson, William;Rodeo, Scott A.
通讯作者：
Rodeo, Scott A.
DOI：
10.2214/ajr.08.2256
发表时间：
2009-11
期刊：
AJR. American journal of roentgenology
影响因子：
--
作者：
Friedrich KM;Shepard T;de Oliveira VS;Wang L;Babb JS;Schweitzer M;Regatte R
通讯作者：
Regatte R
影响因子：
19.7
作者：
Pauli, Chantal;Bae, Won C.;Du, Jiang
通讯作者：
Du, Jiang
影响因子：
3.3
作者：
GOLD, GE;PAULY, JM;HERFKENS, RJ
通讯作者：
HERFKENS, RJ
影响因子：
3.8
作者：
McDermott, ID;Richards, SW;Amis, AA
通讯作者：
Amis, AA