The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering.
The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering.
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DOI：
10.1007/s00253-015-6445-0
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发表时间：
2015-06
影响因子：
5
通讯作者：
Davalos, Rafael V.
中科院分区：
文献类型：
--
作者：
Baah-Dwomoh, Adwoa;Rolong, Andrea;Gatenholm, Paul;Davalos, Rafael V.
这项工作研究了使用不可逆电穿孔 (IRE) 通过细菌菌株木糖醋杆菌生物制造 3D 纤维素纳米纤维网络的可行性。 IRE 使用电脉冲通过改变跨膜电位来增加膜通透性；超过阈值，对细胞的损害变得太大并导致细胞死亡。我们假设，使用 IRE 在特定位置和特定时间杀死细菌，我们可以通过局部阻止纤维素生物合成，在整个支架中引入导管。通过数学模型和实验技术，研究了电效应并确定了木糖醋杆菌的IRE参数。我们发现，对于一组特定的参数，施加 8 kV/cm 至 12.5 kV/cm 的电场足以杀死细菌并产生局部孔隙。我们还发现，8 kV/cm 至 12.5 kV/cm 的外加电场（产生 3 kV/cm 的局部电场）足以杀死大多数细菌并产生局部孔隙，但需要 17.5 kV/cm 的外加电场才能杀死所有细菌。结果表明，IRE 可能是为骨科应用创建具有适当孔隙率的支架的有效工具。理想情况下，这些工程支架可用于成功治疗骨软骨缺陷。
This work investigates the feasibility of the use of irreversible electroporation (IRE) in the biofabrication of 3D cellulose nanofibril networks via the bacterial strain Gluconacetobacter xylinus. IRE uses electrical pulses to increase membrane permeability by altering the transmembrane potential; past a threshold, damage to the cell becomes too great and leads to cell death. We hypothesized that using IRE to kill the bacteria at specific locations and particular times, we could introduce conduits in the overall scaffold by preventing cellulose biosynthesis locally. Through mathematical modeling and experimental techniques, electrical effects were investigated and the parameters for IRE of Gluconacetobacter xylinus were determined. We found that for a specific set of parameters, an applied electric field of 8 kV/cm to 12.5 kV/cm was sufficient to kill bacteria and create a localized pore. We also found that an applied electric field of 8 kV/cm to 12.5 kV/cm, which produces a local field of 3 kV/cm was sufficient to kill most of the bacteria and produce a localized pore, but an applied electric field of 17.5 kV/cm was required to kill all. Results suggest that IRE may be an effective tool to create scaffolds with appropriate porosity for orthopedic applications. Ideally, these engineered scaffolds could be used to successfully treat osteochondral defects.
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影响因子：
3.7
作者：
Maglietti F;Michinski S;Olaiz N;Castro M;Suárez C;Marshall G
通讯作者：
Marshall G
影响因子：
2.8
作者：
Garcia, P. A.;Pancotto, T.;Davalos, R. V.
通讯作者：
Davalos, R. V.
影响因子：
8.8
作者：
Gusbeth, Christian;Frey, Wolfgang;Bluhm, Hansjoachim
通讯作者：
Bluhm, Hansjoachim
影响因子：
3.8
作者：
Neal, Robert E., II;Singh, Ravi;Hatcher, Heather C.;Kock, Nancy D.;Torti, Suzy V.;Davalos, Rafael V.
通讯作者：
Davalos, Rafael V.
影响因子：
3.4
作者：
Gimsa, J;Wachner, D
通讯作者：
Wachner, D