Phasic Activation of Dorsal Raphe Serotonergic Neurons Increases Pupil Size.
Phasic Activation of Dorsal Raphe Serotonergic Neurons Increases Pupil Size.
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DOI：
10.1016/j.cub.2020.09.090
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发表时间：
2021-01-11
期刊：
影响因子：
--
通讯作者：
Mainen ZF
中科院分区：
文献类型：
--
作者：
Cazettes F;Reato D;Morais JP;Renart A;Mainen ZF
在恒定亮度下，瞳孔大小(PS)的瞬时变化与唤醒状态的快速变化相关联，这被解释为警觉、突显或意外信号。这种波动的神经控制可能涉及多个脑区和神经调节系统，但它通常与去甲肾上腺素系统的时相活动有关。5-羟色胺(5-HT)是一种神经调节剂，也与觉醒有关，如睡眠-觉醒转换、动机状态调节和意外事件的信号传递，似乎影响PS，但这些影响尚未被详细研究。在这里，我们发现，5-羟色胺神经元的时相刺激引起一过性PS改变。我们使用光遗传激活5-羟色胺神经元在执行觅食任务的头部固定的小鼠的中缝背核(DRN)。5-羟色胺驱动的PS调制在整个光刺激期间保持不变，并在刺激结束后持续几秒钟。我们没有发现5-羟色胺神经元激活导致PS增加的证据，这是光刺激与行为变量(如运动或舔舔)相互作用的结果。此外，我们观察到5-羟色胺对PS的影响依赖于环境不确定性的水平，这与5-羟色胺可能报告意外信号的想法一致。这些结果促进了我们对PS的神经调节控制的理解，揭示了5-羟色胺神经元的时相激活与PS的变化之间的密切关系。5-羟色胺神经元的时相性光发生激活增加了瞳孔大小，5-羟色胺神经元激活的影响不是行为状态所特有的，5-羟色胺对瞳孔大小的影响与不确定性程度有关，5-羟色胺(5-HT)对瞳孔大小的影响尚未被详细研究。Cazettes等人。结果表明，5-羟色胺神经元的相位性光遗传激活会导致执行觅食任务的小鼠的瞳孔大小发生变化。5-羟色胺对瞳孔大小的影响并不特定于行为变量，但似乎与任务统计有关。
Transient variations in pupil size (PS) under constant luminance are coupled to rapid changes in arousal state, which have been interpreted as vigilance, salience, or a surprise signal. Neural control of such fluctuations presumably involves multiple brain regions and neuromodulatory systems, but it is often associated with phasic activity of the noradrenergic system. Serotonin (5-HT), a neuromodulator also implicated in aspects of arousal such as sleep-wake transitions, motivational state regulation, and signaling of unexpected events, seems to affect PS, but these effects have not been investigated in detail. Here we show that phasic 5-HT neuron stimulation causes transient PS changes. We used optogenetic activation of 5-HT neurons in the dorsal raphe nucleus (DRN) of head-fixed mice performing a foraging task. 5-HT-driven modulations of PS were maintained throughout the photostimulation period and sustained for a few seconds after the end of stimulation. We found no evidence that the increase in PS with activation of 5-HT neurons resulted from interactions of photostimulation with behavioral variables, such as locomotion or licking. Furthermore, we observed that the effect of 5-HT on PS depended on the level of environmental uncertainty, consistent with the idea that 5-HT could report a surprise signal. These results advance our understanding of the neuromodulatory control of PS, revealing a tight relationship between phasic activation of 5-HT neurons and changes in PS. Phasic optogenetic activation of DRN 5-HT neurons increases pupil size The effects of 5-HT neuron activation are not specific to behavioral states The effects of 5-HT on pupil size are linked to the level of uncertainty The effects of serotonin (5-HT) on pupil size have not been investigated in detail. Cazettes et al. show that phasic optogenetic activation of 5-HT neurons causes pupil size changes in mice performing a foraging task. The 5-HT effects on pupil size are not specific to behavioral variables but appear to be linked to the task statistics.
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16.6
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