Phylogenetic Implication of Large Intergenic Spacers: Insights from a Mitogenomic Comparison of Prosopocoilus Stag Beetles (Coleoptera: Lucanidae).
Phylogenetic Implication of Large Intergenic Spacers: Insights from a Mitogenomic Comparison of Prosopocoilus Stag Beetles (Coleoptera: Lucanidae).
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大基因间间隔的系统发育意义：Prosopocoilus 锹虫（鞘翅目：Lucanidae）线粒体基因组比较的见解
DOI：
10.3390/ani12131595
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发表时间：
2022-06-21
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影响因子：
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昆虫线粒体基因组在某些谱系中表现出高度的多样性。在一些鞘翅目物种的有丝分裂基因组中，一个大的基因间间隔区（IGS）已被确定。然而，很少有人知道的有丝分裂基因组的鲁卡尼甲虫。为了进一步了解绿盲蝽属（Prosopocoilus）的种间亲缘关系，对两种绿盲蝽（Prosopocoilus castaneus和Prosopocoilus laterotarsus）进行了新的序列测定和比较分析。值得注意的是，这两个新测序的Prosopocoilus物种包含一个大的IGS位于trnI和trnQ之间。我们的基因组分析表明，栗和P. laterotarsus聚在一个分支与典型的Prosopocoilus物种（Prosopocoilus confucius，Prosopocoilus blanchardi，和Prosopocoilus astacoides）。这些结果为进一步研究该属植物的系统发育关系提供了有价值的数据。为探讨拟绒螯蟹属（Prosopocoilus）有丝分裂基因组的特征及系统发育关系，对拟绒螯蟹（Prosopocoilus）和侧跗拟绒螯蟹（Prosopocoilus laterotarsus）的有丝分裂基因组进行了新的测序和比较分析。在这两个Lucanid甲虫的有丝分裂基因组的安排是相同的，在推断的祖先昆虫，和其他Lucanids的核苷酸组成是高度偏向A + T。13个蛋白质编码基因（PCG）的进化速率表明，它们的进化是基于纯化选择。值得注意的是，我们发现trnI和trnQ基因之间存在大的IGS的证据，其长度从375 bp（在栗树中）到158 bp（在侧跗树中）不等。在大的IGS区域内，发现短序列（TAAAA）在这两个物种中是独特的，这提供了对哺乳动物基因组重建的见解。使用最大似然法（IQ-TREE）和贝叶斯法（PhyloBayes）对来自已发表的有丝分裂基因组（n = 29）的核苷酸和氨基酸（AA）中的13个蛋白质编码基因（PCG）进行系统发育分析。Prosopocoilus属被发现构成一个独特的分支与高节点的支持。总的来说，我们的研究结果表明，大IGS的特征分析（存在或不存在，大小和位置）在属Prosopocoilus的有丝分裂基因组可能是信息的系统发育和分类学分析和属Prosopocoilus的评价，尽管密集的采样材料需要。
Insect mitochondrial genomes (mitogenomes) show high diversity in some lineages. In the mitogenome of some Coleoptera species, a large intergenic spacer (IGS) has been identified. However, very little is known about mitogenomes of lucanid beetles. In this work, to provide further insight into the phylogenic relationships among species in lucanid beetles (genus Prosopocoilus), two Prosopocoilus species (Prosopocoilus castaneus and Prosopocoilus laterotarsus) were newly sequenced and comparatively analyzed. Significantly, the two newly sequenced Prosopocoilus species contained a large IGS located between trnI and trnQ. Our phylogenomic analyses showed that P. castaneus and P. laterotarsus were clustered in a clade with typical Prosopocoilus species (Prosopocoilus confucius, Prosopocoilus blanchardi, and Prosopocoilus astacoides). These results provide valuable data for the future study of the phylogenetic relationships in this genus. To explore the characteristics of mitogenomes and discuss the phylogenetic relationships within the genus Prosopocoilus, the mitogenomes of two species (P. castaneus and P. laterotarsus) were newly sequenced and comparatively analyzed. The arrangement of the mitogenome in these two lucanid beetles was the same as that in the inferred ancestral insect, and the nucleotide composition was highly biased towards A + T as in other lucanids. The evolutionary rates of 13 protein-coding genes (PCGs) suggested that their evolution was based on purifying selection. Notably, we found evidence of the presence of a large IGS between trnI and trnQ genes, whose length varied from 375 bp (in P. castaneus) to 158 bp (in P. laterotarsus). Within the large IGS region, a short sequence (TAAAA) was found to be unique among these two species, providing insights into phylogenomic reconstruction. Phylogenetic analyses were performed using the maximum likelihood (IQ-TREE) and Bayesian (PhyloBayes) methods based on 13 protein-coding genes (PCGs) in nucleotides and amino acids (AA) from published mitogenomes (n = 29). The genus Prosopocoilus was found to constitute a distinct clade with high nodal support. Overall, our findings suggested that analysis of the characteristics of the large IGS (presence or absence, size, and location) in mitogenomes of the genus Prosopocoilus may be informative for the phylogenetic and taxonomic analyses and for evaluation of the genus Prosopocoilus, despite the dense sampling materials needed.
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影响因子：
4.4
作者：
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