Association of Atopobium vaginae, a recently described metronidazole resistant anaerobe, with bacterial vaginosis.
Association of Atopobium vaginae, a recently described metronidazole resistant anaerobe, with bacterial vaginosis.
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DOI：
10.1186/1471-2334-4-5
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发表时间：
2004-02-13
影响因子：
3.7
通讯作者：
Martin DH
中科院分区：
文献类型：
--
作者：
Ferris MJ;Masztal A;Aldridge KE;Fortenberry JD;Fidel PL Jr;Martin DH
细菌性阴道病（BV）是一种多微生物综合征，其特征是阴道植物群发生变化，主要由乳杆菌属细菌引起。BV的原因尚不清楚，但这种情况与各种医疗结果有关。阴道奇异菌（Atopobium vaginae）最近才被发现。它不容易被商业诊断试剂盒识别。其临床意义尚不清楚，但最近已从输卵管卵巢脓肿中分离出来。采用变性梯度凝胶电泳（DGGE）将PCR扩增的16 S rRNA基因片段在聚丙烯酰胺凝胶上的泳道内分离成条带，并对其进行核苷酸测序，以检查46例临床上描述为正常（乳杆菌属）的患者的细菌阴道植物群。占优势; Nugent评分≤ 3）和异常植物群（Nugent评分≥ 4）。这些妇女的年龄从14岁到48岁不等，82%是非洲裔美国人。正常和BV阳性患者的DGGE带型明显不同。正常患者的那些包含1至4条集中在凝胶泳道中心区域的条带，而BV阳性患者的那些包含并非全部集中在凝胶泳道中心区域的条带。进一步的分析表明，大多数BV阳性患者（12/22）出现阴道变形菌带，而正常患者中相应的带很少（2/24）。（P < 0.001）两个A.从DGGE分析表明存在这种微生物的两名患者中培养阴道分离物。两个A。阴道16 S rRNA基因序列分析。从相应的阴道植物群的DGGE条带中获得了相同的两个序列。序列在用于DGGE分析的短片段（~300 bp）上相差一个核苷酸，并在变性梯度凝胶中迁移到略微不同的点。两株分离株均为严格厌氧菌，对甲硝唑高度耐药。结果表明，A.阴道可能是构成异常阴道植物群的复杂细菌生态的重要组成部分。如果进一步的研究证实这种微生物始终对甲硝唑耐药，则它可能在治疗失败中发挥作用。
Bacterial vaginosis (BV) is a polymicrobial syndrome characterized by a change in vaginal flora away from predominantly Lactobacillus species. The cause of BV is unknown, but the condition has been implicated in diverse medical outcomes. The bacterium Atopobium vaginae has been recognized only recently. It is not readily identified by commercial diagnostic kits. Its clinical significance is unknown but it has recently been isolated from a tuboovarian abcess. Nucleotide sequencing of PCR amplified 16S rRNA gene segments, that were separated into bands within lanes on polyacrylamide gels by denaturing gradient gel electrophoresis (DGGE), was used to examine bacterial vaginal flora in 46 patients clinically described as having normal (Lactobacillus spp. predominant; Nugent score ≤ 3) and abnormal flora (Nugent score ≥ 4). These women ranged in age from 14 to 48 and 82% were African American. The DGGE banding patterns of normal and BV-positive patients were recognizably distinct. Those of normal patients contained 1 to 4 bands that were focused in the centre region of the gel lane, while those of BV positive patients contained bands that were not all focused in the center region of the gel lane. More detailed analysis of patterns revealed that bands identified as Atopobium vaginae were present in a majority (12/22) of BV positive patients, while corresponding bands were rare (2/24) in normal patients. (P < 0.001) Two A. vaginae isolates were cultivated from two patients whose DGGE analyses indicated the presence of this organism. Two A. vaginae 16S rRNA gene sequences were identified among the clinical isolates. The same two sequences were obtained from DGGE bands of the corresponding vaginal flora. The sequences differed by one nucleotide over the short (~300 bp) segment used for DGGE analysis and migrated to slightly different points in denaturing gradient gels. Both isolates were strict anaerobes and highly metronidazole resistant. The results suggest that A. vaginae may be an important component of the complex bacterial ecology that constitutes abnormal vaginal flora. This organism could play a role in treatment failure if further studies confirm it is consistently metronidozole resistant.
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影响因子：
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作者：
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通讯作者：
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