Expression of miRNA-106b in conventional renal cell carcinoma is a potential marker for prediction of early metastasis after nephrectomy.
Expression of miRNA-106b in conventional renal cell carcinoma is a potential marker for prediction of early metastasis after nephrectomy.
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DOI：
10.1186/1756-9966-29-90
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发表时间：
2010-07-07
期刊：
影响因子：
--
通讯作者：
Vyzula R
中科院分区：
文献类型：
--
作者：
Slaby O;Jancovicova J;Lakomy R;Svoboda M;Poprach A;Fabian P;Kren L;Michalek J;Vyzula R
MicroRNAs是内源性表达的非编码调控RNA。先前的研究表明，几种microRNAs在肾癌中的表达水平发生了变化。应用TaqMan实时定量聚合酶链式反应技术检测38例常规肾细胞癌(RCC)和10例非肿瘤性肾实质组织中microRNAs的表达水平。在肿瘤组织中，miRNA-155(p<；0.0001)、miRNA-210(p<；0.0001)、miRNA-106a(p<；0.0001)和miRNA-106b(p<；0.0001)的表达水平显著升高，而miRNA-141(p<；0.0001)和miRNA-200c(p<；0.0001)的表达水平显著降低。MiRNA-182和miRNA-200b在肿瘤组织和肾实质中的表达水平差异无统计学意义。我们的数据表明，在发生转移的患者中，miRNA-106b的表达水平显著降低(p=0.030)，miR-106b是肾癌患者肾切除术后早期转移的潜在预测标志物(长序p=0.032)。我们已经证实了之前使用标准化实时荧光PCR方法进行miRNA微阵列分析所获得的观察结果。我们第一次确定了miRNA-106b的预后意义，在更大的患者组上验证后，它可能是一种有希望的肾癌患者的生物标记物。
MicroRNAs are endogenously expressed regulatory noncoding RNAs. Previous studies have shown altered expression levels of several microRNAs in renal cell carcinoma. We examined the expression levels of selected microRNAs in 38 samples of conventional renal cell carcinoma (RCC) and 10 samples of non-tumoral renal parenchyma using TaqMan real-time PCR method. The expression levels of miRNA-155 (p < 0.0001), miRNA-210 (p < 0.0001), miRNA-106a (p < 0.0001) and miRNA-106b (p < 0.0001) were significantly over-expressed in tumor tissue, whereas the expression of miRNA-141 (p < 0.0001) and miRNA-200c (p < 0.0001) were significantly decreased in RCC samples. There were no significant differences between expression levels of miRNA-182 and miRNA-200b in tumor samples and renal parenchyma. Our data suggest that expression levels of miRNA-106b are significantly lower in tumors of patients who developed metastasis (p = 0.030) and miR-106b is a potential predictive marker of early metastasis after nephrectomy in RCC patients (long-rank p = 0.032). We have confirmed previous observations obtained by miRNA microarray analysis using standardized real-time PCR method. For the first time, we have identified a prognostic significance of miRNA-106b, which, after validation on a larger group of patients, maybe useful as a promising biomarker in patients with RCC.
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影响因子：
12.3
作者：
Galasso M;Sana ME;Volinia S
通讯作者：
Volinia S
DOI：
10.1111/j.1440-1746.2008.05666.x
发表时间：
2009-04-01
影响因子：
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作者：
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影响因子：
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作者：
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