TAC3/TACR3 System Function in the Catadromous Migration Teleost, Anguilla japonica.
TAC3/TACR3 System Function in the Catadromous Migration Teleost, Anguilla japonica.
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DOI：
10.3389/fendo.2022.848808
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发表时间：
2022
影响因子：
5.2
通讯作者：
Qi, Xin
中科院分区：
文献类型：
--
作者：
Zuo, Chenpeng;Lyu, Likang;Zou, Wenhui;Wen, Haishen;Li, Yun;Qi, Xin
神经激肽B（NKB）是速激肽（TAC）家族的成员之一，在哺乳动物神经肽分泌中起重要作用，与生殖有关。然而，其在产卵迁移硬骨鱼的潜在作用不太清楚。本研究以日本鳗鲡（Anguilla japonica）为试验材料，研究了NKB对鳗鲡繁殖的调控作用。结果表明，在日本鳗鲡中发现了2个tac 3基因和1个tacr 3基因。序列分析表明，两个tac 3转录本，tac 3a和tac 3b，编码四个NKB：NKBa-13，NKBa-10，NKBb-13和NKBb-10。然而，与其他物种相比，TACR 3蛋白的突变引起的早期终止被证实，导致35个氨基酸（aa）的受体的C-末端的损失。在不同组织中的表达分析表明，tac 3a和tac 3b mRNA在脑中高度表达。原位杂交将tac 3a和tac 3b mRNA定位于几个大脑区域，主要是端脑和下丘脑。由于日本鳗鲡TACR 3的突变，我们进一步分析了它是否能够激活下游信号通路。荧光素酶检测结果表明，日本鳗鲡NKBs对cAMP反应元件（CRE）和甾醇反应元件（SRE）信号通路具有负调控作用。腹腔内注射100 ng/g的四种不同的NKB成熟肽对gnrh或gth基因表达有负面影响。而高浓度NKBa-10和NKBb-13（1,000 ng/g）可显著上调mgnrh和fshb或lhb的表达水平，这可能是由其他受体介导的。因此，NKBs/NK 3Rs系统在调控鳗鲡青春期启动中具有重要作用。
Neurokinin B (NKB), a member of the tachykinin (TAC) family, plays important roles in mammalian neuropeptide secretion in related to reproduction. However, its potential role in spawning migration teleost is less clear. In the present study, Japanese eel (Anguilla japonica) was employed to study the performance of NKB in regulating reproduction. Results showed that two tac3 and one tacr3 genes were identified in Japanese eel. Sequence analysis showed that two tac3 transcripts, tac3a and tac3b, encode four NKBs: NKBa-13, NKBa-10, NKBb-13, and NKBb-10. However, compared with other species, a mutation caused early termination of TACR3 protein was confirmed, leading to the loss of the 35 amino acid (aa) C-terminal of the receptor. Expression analysis in different tissues showed that both tac3a and tac3b mRNAs were highly expressed in the brain. In situ hybridization localized both tac3a and tac3b mRNAs to several brain regions, mainly in the telencephalon and hypothalamus. Because of the mutation in TACR3 of Japanese eel, we further analyzed whether it could activate the downstream signaling pathway. Luciferase assay results showed the negative regulation of cAMP Response Element (CRE) and Sterol Response Element (SRE) signal pathways by Japanese eel NKBs. Intraperitoneal injection of four different NKB mature peptides at 100 ng/g had negative effect on either gnrh or gth gene expression. However, the high concentration of NKBa-10 and NKBb-13 (1,000 ng/g) upregulated mgnrh and fshb or lhb expression level significantly, which may be mediated by other receptors. In general, the NKBs/NK3Rs system has important functions in regulating eel puberty onset.
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影响因子：
5.2
作者：
Campo A;Lafont AG;Lefranc B;Leprince J;Tostivint H;Kamech N;Dufour S;Rousseau K
通讯作者：
Rousseau K
DOI：
10.1073/pnas.1119165109
发表时间：
2012-06-26
影响因子：
11.1
作者：
Biran, Jakob;Palevitch, Ori;Levavi-Sivan, Berta
通讯作者：
Levavi-Sivan, Berta
影响因子：
3.3
作者：
Ciofi, P.;Leroy, D.;Tramu, G.
通讯作者：
Tramu, G.
DOI：
10.1051/rnd:2005019
发表时间：
2005-05-01
期刊：
REPRODUCTION NUTRITION DEVELOPMENT
影响因子：
--
作者：
Jalabert, B
通讯作者：
Jalabert, B
影响因子：
2.9
作者：
Dahl, Stephanie K.;Amstalden, Marcel;Lehman, Michael
通讯作者：
Lehman, Michael