Decreased urothelial expression of caveolin 1 and 2 in aging rats showing detrusor overactivity: Potential association with aging bladder.
Decreased urothelial expression of caveolin 1 and 2 in aging rats showing detrusor overactivity: Potential association with aging bladder.
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DOI：
10.4111/icu.20210284
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发表时间：
2021-11
影响因子：
2.3
通讯作者：
Kim SO
中科院分区：
文献类型：
--
作者：
Kim JH;Yu SH;Kim SO
本研究旨在探讨衰老对大鼠膀胱功能及尿路上皮小窝蛋白表达的影响。雌性Sprague-Dawley大鼠分为两组：幼年对照组（12周）和老年组（80周）。采用尿动力学测量比较两组间的收缩间隔和收缩压力。应用Western blot和免疫荧光显微镜检测大鼠膀胱尿路上皮中小窝蛋白1和2的表达及细胞定位。老年组收缩间隔（3.7±0.5 min）明显短于年轻对照组（6.2±0.8 min）。老年组的平均收缩压（mmHg）（8.4±0.6 mmHg）低于年轻对照组（13.2±1.3 mmHg）。小窝蛋白1和2表达于尿路上皮上皮下区。老年组小窝蛋白1和小窝蛋白2的蛋白表达均明显低于年轻对照组。衰老引起大鼠膀胱尿路上皮小窝蛋白1和2的表达发生显著变化。这些发现表明，这些分子可能在与衰老相关的膀胱功能变化中具有特定的作用。
The purpose of this study was to investigate the effect of aging on bladder function and caveolin protein expression in rat urothelium. Female Sprague-Dawley rats were divided into the following two groups: young age control group (12 weeks) and old-aged group of rats (80 weeks). Urodynamic measurements were taken to compare the contraction interval and the contraction pressure between the two groups. The expression and cellular localization of caveolin 1 and 2 in the urothelium of the rat urinary bladder were determined by Western blot and immunofluorescence microscopy. In cystometrograms, the contraction interval (min) was significantly shorter in the old-aged group (3.7±0.5 min) than in the young age control group (6.2±0.8 min). Also, the average contraction pressure (mmHg) was lower in the old-aged group (8.4±0.6 mmHg) than in the young age control group (13.2±1.3 mmHg). Caveolin 1 and 2 were expressed in the subepithelial area in the urothelium. The protein expression of both caveolin 1 and 2 was significantly lower in the old-aged group than in the young age control group. Aging caused a significant change in the expression of caveolin 1 and 2 in the urothelium of the rat urinary bladder. These findings suggest that these molecules might have specific roles in the functional change of the urinary bladder that occurs in association with aging.
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影响因子：
3.2
作者：
PALADE, GE
通讯作者：
PALADE, GE
影响因子：
10.8
作者：
Ratajczak, P;Damy, T;Samuel, JL
通讯作者：
Samuel, JL
影响因子：
2
作者：
Cristofaro, V.;Peters, C. A.;Sullivan, M. P.
通讯作者：
Sullivan, M. P.
影响因子：
2
作者：
Kim, Sun-Ouck;Song, Seung Hee;Park, Kwangsung
通讯作者：
Park, Kwangsung
影响因子：
6.6
作者：
ELBADAWI, A;YALLA, SV;RESNICK, NM
通讯作者：
RESNICK, NM