Human Umbilical Cord MSCs as New Cell Sources for Promoting Periodontal Regeneration in Inflammatory Periodontal Defect.
Human Umbilical Cord MSCs as New Cell Sources for Promoting Periodontal Regeneration in Inflammatory Periodontal Defect.
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人脐带间充质干细胞作为促进炎症性牙周缺损牙周再生的新细胞来源
作者：
Shang F;Liu S;Ming L;Tian R;Jin F;Ding Y;Zhang Y;Zhang H;Deng Z;Jin Y
人牙周膜干细胞（hPDLSCs）移植是牙周再生的一种有前途的方法，然而，由于细胞分离所需的侵入性程序，细胞来源是有限的。由于人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hUCMSCs）可以廉价且取之不尽地获得，因此我们将hUCMSCs的再生潜能与hPDLSCs进行比较，以确定hUCMSCs是否可以作为牙周再生的新细胞来源。方法以人牙周膜干细胞为对照，检测人脐带间充质干细胞的多向分化能力和抗炎活性。我们分别使用hUCMSC和hPDLSC构建细胞聚集体（CA）。将hPDLSCs-CA和hUCMSCs-CA分别与β-磷酸三钙生物陶瓷（β-TCP）复合，在大鼠牙周炎性缺损模型中检测其再生能力。结果hPDLSCs的成骨分化潜能高于hUCMSCs。同时，hUCMSCs比hPDLSCs具有更强的细胞外基质分泌和抗炎能力。与hPDLSCs相似，hUCMSCs能够在炎症性牙周炎条件下促进牙周软硬组织的再生。hPDLSCs组和hUCMSCs组新生骨和牙周韧带的数量明显多于未处理组。结论：hUCMSCs与hPDLSCs对牙周组织的再生具有相似的促进作用，可作为牙周组织再生的新细胞来源。
Human periodontal ligament stem cells (hPDLSCs) transplantation represents a promising approach for periodontal regeneration; however, the cell source is limited due to the invasive procedure required for cell isolation. As human umbilical cord mesenchymal stem cells (hUCMSCs) can be harvested inexpensively and inexhaustibly, here we evaluated the regenerative potentials of hUCMSCs as compared with hPDLSCs to determine whether hUCMSCs could be used as new cell sources for periodontal regeneration. Methods The characteristics of hUCMSCs, including multi-differentiation ability and anti-inflammatory capability, were determined by comparison with hPDLSCs. We constructed cell aggregates (CA) using hUCMSCs and hPDLSCs respectively. Then hPDLSCs-CA and hUCMSCs-CA were combined with β-tricalcium phosphate bioceramic (β-TCP) respectively and their regenerative potentials were determined in a rat inflammatory periodontal defect model. Results hPDLSCs showed higher osteogenic differentiation potentials than hUCMSCs. Meanwhile, hUCMSCs showed higher extracellular matrix secretion and anti-inflammatory abilities than hPDLSCs. Similar to hPDLSCs, hUCMSCs were able to contribute to regeneration of both soft and hard periodontal tissues under inflammatory periodontitis condition. There were more newly formed bone and periodontal ligaments in hPDLSCs and hUCMSCs groups than in non-cell treated group. Moreover, no significant differences of regenerative promoting effects between hPDLSCs and hUCMSCs were found. Conclusion: hUCMSCs generated similar promoting effects on periodontal regeneration compared with hPDLSCs, and can be used as new cell sources for periodontal regeneration.
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影响因子：
6.1
作者：
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