The Mitogen-Activated Protein Kinase CgMK1 Governs Appressorium Formation, Melanin Synthesis, and Plant Infection of Colletotrichum gloeosporioides.
The Mitogen-Activated Protein Kinase CgMK1 Governs Appressorium Formation, Melanin Synthesis, and Plant Infection of Colletotrichum gloeosporioides.
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丝裂原激活蛋白激酶 CgMK1 控制粘孢炭疽菌的附着胞形成、黑色素合成和植物感染
DOI：
10.3389/fmicb.2017.02216
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发表时间：
2017
影响因子：
5.2
通讯作者：
Tian C
中科院分区：
文献类型：
--
作者：
He P;Wang Y;Wang X;Zhang X;Tian C
真菌Colletotrichum gloeosporiodes用一种称为附着胞的特化细胞感染植物宿主，附着胞是黑化的，是穿透植物细胞壁所必需的。在这里，我们表明，丝裂原活化蛋白激酶CgMK 1控制附着胞的形成和毒性的白杨炭疽真菌C。胶孢菌CgMK 1的缺失损害气生菌丝生长和生物量积累，并且CgMK 1负责黑色素生物合成相关基因的表达。CgMK 1缺失突变体无法形成附着胞，并失去在受伤或未受伤的白杨叶片上定殖的能力，导致毒力丧失。我们表明，外源性应用的cAMP不能恢复有缺陷的附着胞形成的CgMK1缺失突变体，这表明CgMK1可能发挥作用的下游或独立的cAMP依赖的信号附着胞形成。此外，CgMK 1突变体对高渗透敏感，表明CgMK 1在胁迫响应中起重要作用。CgMK 1在调节附着胞的形成、黑色素的合成和毒力等方面起着重要作用。胶孢子虫
The fungus Colletotrichum gloeosporiodes infects plant hosts with a specialized cell called an appressorium, which is melanized and required for plant cell wall penetration. Here, we show that the mitogen-activated protein kinase CgMK1 governs appressorium formation and virulence in the poplar anthracnose fungus C. gloeosporioides. Deletion of CgMK1 impairs aerial hyphal growth and biomass accumulation, and CgMK1 is responsible for the expression of melanin biosynthesis-associated genes. CgMK1 deletion mutants are unable to form appressorium and lose the capacity to colonize either wounded or unwounded poplar leaves, leading to loss of virulence. We demonstrate that the exogenous application of cAMP fails to restore defective appressorium formation in the CgMK1 deletion mutants, suggesting that CgMK1 may function downstream or independent of a cAMP-dependent signal for appressorium formation. Moreover, CgMK1 mutants were sensitive to high osmosis, indicating that CgMK1 plays an important role in stress response. We conclude that CgMK1 plays a vital role in regulating appressorium formation, melanin biosynthesis, and virulence in C. gloeosporiodes.
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影响因子：
3.7
作者：
Leng Y;Zhong S
通讯作者：
Zhong S
影响因子：
3.7
作者：
Li G;Zhou X;Kong L;Wang Y;Zhang H;Zhu H;Mitchell TK;Dean RA;Xu JR
通讯作者：
Xu JR
DOI：
10.1016/0147-5975(89)90036-4
发表时间：
1989-12-01
期刊：
EXPERIMENTAL MYCOLOGY
影响因子：
--
作者：
HOWARD, RJ;FERRARI, MA
通讯作者：
FERRARI, MA
影响因子：
2.5
作者：
Rauyaree, P;Ospina-Giraldo, MD;Dobinson, KF
通讯作者：
Dobinson, KF
影响因子：
4.9
作者：
Leroch, Michaela;Mueller, Nathalie;Hahn, Matthias
通讯作者：
Hahn, Matthias