Mechanism of thyroid hormone signaling in skeletal muscle of aging mice
Mechanism of thyroid hormone signaling in skeletal muscle of aging mice
复制标题
衰老小鼠骨骼肌中甲状腺激素信号传导机制
DOI：
10.1007/s12020-020-02428-9
复制
发表时间：
2020-07
期刊：
影响因子：
3.7
通讯作者：
Duan Y
中科院分区：
文献类型：
--
作者：
Wang L;Sheng Y;Xu W;Sun M;Lv S;Yu J;Wang X;Ding G;Duan Y
背景与目的骨骼肌（SM）是甲状腺激素（TH）作用的靶点.然而，TH信号在老年SM中的状态仍不清楚。方法将30只C57 BL/6 J雄性小鼠按不同月龄分为6、15和22月龄组（6、15和22月龄组）。通过分析天平、握力测试和组织学分析评估物理参数。采用酶联免疫吸附法检测甲状腺功能。结果SM中sp16、p21和p53 mRNA表达水平随年龄增长而升高。与6和15 M组相比，22 M组的肌肉重量和强度降低。22 M小鼠的甲状腺激素浓度，包括游离三碘甲状腺原氨酸（FT 3），游离甲状腺素（FT 4）和促甲状腺激素（TSH），与6 M或15 M小鼠相比，没有显示出显着差异，虽然FT 3显示略有下降，TSH出现轻度增加，随着年龄的增长。与其他组相比，22 M组TH转运体（包括MCT 8和MCT 10）以及碘甲腺原氨酸脱碘酶2型（DIO 2）和3型（DIO 3）的mRNA水平较高，而TH受体α（TRα）mRNA和蛋白表达较低。I型肌球蛋白重链（MyHC I），MyHC IIx，和MyHC IIa的上调和IIb型MyHC（MyHC IIb）下调SM随着aging.ConclusionsTH信号在SM的变化与老化。
Background and aimSkeletal muscle (SM) has been shown as a target of thyroid hormones (THs). However, the status of TH signaling in aged SM remains unclear. This study aimed to explore the mechanism of TH signaling in SM of aging mice.MethodsThirty C57BL/6J male mice were divided into 6-, 15- and 22-month (6, 15 and 22M) groups according to different age. Physical parameters were evaluated by analytical balance, grip strength test and histological analysis. Thyroid function was detected by enzyme-linked immunosorbent assay. TH signaling was compared among the three groups by real-time PCR and western blotting analysis.Resultsp16, p21, and p53 mRNA levels in SM increased in age-dependent manner. The muscle weight and strength decreased in 22M group compared to 6 and 15M groups. Concentrations of thyroid hormones, including free triiodothyronine (FT3), free thyroxine (FT4) and thyroid-stimulating hormone (TSH) in 22 M mice were not shown significant difference compared to 6M or 15M mice, although FT3 showed slightly decrease and TSH appeared a mild increase accompanying with age. mRNA levels of TH transporters, including MCT8 and MCT10, as well as iodothyronine deiodinase type 2 (DIO2) and type 3 (DIO3), were higher in 22M, while TH receptor α (TRα) mRNA and protein expression was lower in 22M, compared to the other groups. Type-I myosin heavy chain (MyHC I), MyHC IIx, and MyHC IIa were upregulated and Type-IIb MyHC (MyHC IIb) was downregulated in SM with advancing age.ConclusionsTH signaling in SM changes with aging.
登录
查看更多内容
心肌和骨骼肌中的 microRNA 和甲状腺激素信号传导
影响因子：
7.5
作者：
Zhang D;Li Y;Liu S;Wang YC;Guo F;Zhai Q;Jiang J;Ying H
通讯作者：
Ying H
影响因子：
4.8
作者：
J. E. Silva;T. Dick;P. Larsen
通讯作者：
J. E. Silva;T. Dick;P. Larsen
影响因子：
--
作者：
Y. Duan;Chao Liu;Shang-yong Feng;Xiao-dong Wang;Wei Tang;X. Mao;Shu-hang Xu;Yu Feng
通讯作者：
Y. Duan;Chao Liu;Shang-yong Feng;Xiao-dong Wang;Wei Tang;X. Mao;Shu-hang Xu;Yu Feng
影响因子：
4.1
作者：
Ambrosio, Raffaele;De Stefano, Maria Angela;Salvatore, Domenico
通讯作者：
Salvatore, Domenico
DOI：
10.1152/ajpregu.2000.278.6.r1545
发表时间：
2000-06
期刊：
American journal of physiology. Regulatory, integrative and comparative physiology
影响因子：
--
作者：
Fushun Yu;Fushun Yu;S. Göthe;Lilian Wikström;Douglas Forrest;Björn Vennström;Lars Larsson;
通讯作者：
Fushun Yu;Fushun Yu;S. Göthe;Lilian Wikström;Douglas Forrest;Björn Vennström;Lars Larsson;