Early vertebrate chromosome duplications and the evolution of the neuropeptide Y receptor gene regions.
Early vertebrate chromosome duplications and the evolution of the neuropeptide Y receptor gene regions.
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DOI：
10.1186/1471-2148-8-184
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发表时间：
2008-06-25
影响因子：
3.4
通讯作者：
Larhammar D
中科院分区：
文献类型：
--
作者：
Larsson TA;Olsson F;Sundstrom G;Lundin LG;Brenner S;Venkatesh B;Larhammar D
在早期脊椎动物进化中扩展的许多基因家族之一是G蛋白偶联受体的神经肽（NPY）受体家族。我们实验室的早期工作表明，在现存的脊椎动物中发现的几个NPY受体基因是在有颌脊椎动物（有颌类）起源之前的两次基因组重复和在actinopterygian谱系中的一次额外的基因组重复的结果，这是基于它们在染色体上的位置共享几个基因家族。在这项研究中，我们已经调查了，在五种脊椎动物的基因组中，45个基因家族的成员接近的NPY受体基因的硬骨鱼的紧凑的基因组中的Tetraodon nigroviridis和红鳍东方鲀。这些对应于智人染色体4，5，8和10。通过系统发育分析，在H. sapiens、M. musculus、D. rerio，T. rubripes和T.黑绿菌属包括NPY受体基因在内的26个基因家族（加上其他实验室最近描述的3个）显示出与早期脊椎动物进化和放线翼动物谱系中的重复一致的树形拓扑结构，从而支持通过区块重复进行扩增。8个基因家族存在妨碍分析的并发症（如短序列长度或可变数量的重复结构域），另外8个家族不支持区块复制（因为这些家族中的旁系同源物似乎起源于另一个时间窗口，而不是拟议的基因组复制事件）。用RT-PCR方法对T. rubripes显示所有5种NPY受体均在脑中表达，Y2、Y4和Y8亚型也在外周器官中表达。我们的结论是，这些基因家族的系统发育分析和染色体定位支持大基因块，甚至整个染色体的重复。因此，这些结果与形成包含人染色体4、5、8和10的旁系同源体的两个早期脊椎动物四倍化和一个硬骨鱼四倍化一致。位置和系统发育数据的组合进一步加强了NPY受体家族中直系同源物和旁系同源物的鉴定。
One of the many gene families that expanded in early vertebrate evolution is the neuropeptide (NPY) receptor family of G-protein coupled receptors. Earlier work by our lab suggested that several of the NPY receptor genes found in extant vertebrates resulted from two genome duplications before the origin of jawed vertebrates (gnathostomes) and one additional genome duplication in the actinopterygian lineage, based on their location on chromosomes sharing several gene families. In this study we have investigated, in five vertebrate genomes, 45 gene families with members close to the NPY receptor genes in the compact genomes of the teleost fishes Tetraodon nigroviridis and Takifugu rubripes. These correspond to Homo sapiens chromosomes 4, 5, 8 and 10. Chromosome regions with conserved synteny were identified and confirmed by phylogenetic analyses in H. sapiens, M. musculus, D. rerio, T. rubripes and T. nigroviridis. 26 gene families, including the NPY receptor genes, (plus 3 described recently by other labs) showed a tree topology consistent with duplications in early vertebrate evolution and in the actinopterygian lineage, thereby supporting expansion through block duplications. Eight gene families had complications that precluded analysis (such as short sequence length or variable number of repeated domains) and another eight families did not support block duplications (because the paralogs in these families seem to have originated in another time window than the proposed genome duplication events). RT-PCR carried out with several tissues in T. rubripes revealed that all five NPY receptors were expressed in the brain and subtypes Y2, Y4 and Y8 were also expressed in peripheral organs. We conclude that the phylogenetic analyses and chromosomal locations of these gene families support duplications of large blocks of genes or even entire chromosomes. Thus, these results are consistent with two early vertebrate tetraploidizations forming a paralogon comprising human chromosomes 4, 5, 8 and 10 and one teleost tetraploidization. The combination of positional and phylogenetic data further strengthens the identification of orthologs and paralogs in the NPY receptor family.
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影响因子：
9.8
作者：
Conant GC;Wolfe KH
通讯作者：
Wolfe KH
影响因子：
4.4
作者：
Dempsey, LA;Li, MJ;Maizels, N
通讯作者：
Maizels, N
影响因子：
4.4
作者：
HART C P;FAINSOD A;RUDDLE F H
通讯作者：
RUDDLE F H
影响因子：
4.1
作者：
Fox, EJ;Stubbs, SA;Wright, SC
通讯作者：
Wright, SC
DOI：
10.1023/a:1022648729770
发表时间：
2003-01-01
期刊：
Journal of Structural and Functional Genomics
影响因子：
--
作者：
Aburomia, Rami;Khaner, Oded;Sidow, Arend
通讯作者：
Sidow, Arend