Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population.
Allelic variation for broad-spectrum resistance and susceptibility to bacterial pathogens identified in a rice MAGIC population.
复制标题
DOI：
10.1111/pbi.12895
复制
发表时间：
2018-02-06
影响因子：
13.8
通讯作者：
Leach JE
中科院分区：
文献类型：
--
作者：
Bossa-Castro AM;Tekete C;Raghavan C;Delorean EE;Dereeper A;Dagno K;Koita O;Mosquera G;Leung H;Verdier V;Leach JE
赋予广谱抗性（BSR）或对多种和不同植物病原体有效的抗性的数量性状基因座（QTL）一直是作物育种计划的难以捉摸的目标。多亲本高世代互交（MAGIC）群体具有多样的遗传组成和高水平的重组，是鉴定BSR QTL的潜在资源。本研究利用水稻MAGIC群体定位了水稻细菌性条斑病（BLS）和白叶枯病（BB）两种主要病害的BSR QTL。oryzicola（Xoc）和稻瘟病菌（Xoo）。控制这些疾病在撒哈拉以南非洲尤其重要，因为目前在该地区部署的品种中没有BSR来源。通过测序对MAGIC建立者和品系进行基因分型，并通过接种Xoc和Xoo的多种菌株在温室和田间进行表型分型。全基因组关联研究（GWAS）和区间作图分析的组合揭示了11个对两种疾病有效的BSR QTL和3个致病变种特异性QTL。最有希望的BSR QTL（qXO-2 - 1、qXO-4 - 1和qXO-11 - 2）赋予对超过9个Xoc和Xoo菌株的抗性。GWAS检测到369个显著的SNP标记，具有可区分的表型效应，允许鉴定赋予抗病性和易感性的等位基因。从长远来看，BSR和感病QTL将提高我们对抗性和感病机制的理解，并将立即成为水稻育种计划的有用资源。
Quantitative trait loci (QTL) that confer broad‐spectrum resistance (BSR), or resistance that is effective against multiple and diverse plant pathogens, have been elusive targets of crop breeding programmes. Multiparent advanced generation intercross (MAGIC) populations, with their diverse genetic composition and high levels of recombination, are potential resources for the identification of QTL for BSR. In this study, a rice MAGIC population was used to map QTL conferring BSR to two major rice diseases, bacterial leaf streak (BLS) and bacterial blight (BB), caused by Xanthomonas oryzae pathovars (pv.) oryzicola (Xoc) and oryzae (Xoo), respectively. Controlling these diseases is particularly important in sub‐Saharan Africa, where no sources of BSR are currently available in deployed varieties. The MAGIC founders and lines were genotyped by sequencing and phenotyped in the greenhouse and field by inoculation with multiple strains of Xoc and Xoo. A combination of genomewide association studies (GWAS) and interval mapping analyses revealed 11 BSR QTL, effective against both diseases, and three pathovar‐specific QTL. The most promising BSR QTL (qXO‐2‐1, qXO‐4‐1 and qXO‐11‐2) conferred resistance to more than nine Xoc and Xoo strains. GWAS detected 369 significant SNP markers with distinguishable phenotypic effects, allowing the identification of alleles conferring disease resistance and susceptibility. The BSR and susceptibility QTL will improve our understanding of the mechanisms of both resistance and susceptibility in the long term and will be immediately useful resources for rice breeding programmes.
登录
查看更多内容
影响因子：
5.4
作者：
Kim, Suk-Man;Suh, Jung-Pil;Jena, Kshirod K.
通讯作者：
Jena, Kshirod K.
影响因子：
5.8
作者：
Huang, B. Emma;George, Andrew W.
通讯作者：
George, Andrew W.
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
DOI：
10.1186/1939-8433-6-11
发表时间：
2013-05-06
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Bandillo N;Raghavan C;Muyco PA;Sevilla MA;Lobina IT;Dilla-Ermita CJ;Tung CW;McCouch S;Thomson M;Mauleon R;Singh RK;Gregorio G;Redoña E;Leung H
通讯作者：
Leung H