Structural basis of membrane bending by the N-BAR protein endophilin.
Structural basis of membrane bending by the N-BAR protein endophilin.
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DOI：
10.1016/j.cell.2012.01.048
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发表时间：
2012-03-30
期刊：
影响因子：
64.5
通讯作者：
Unger VM
中科院分区：
文献类型：
--
作者：
Mim C;Cui H;Gawronski-Salerno JA;Frost A;Lyman E;Voth GA;Unger VM
作为细胞膜曲率调节的关键角色，杆状结构域蛋白通过鲜为人知的机制弯曲双层并招募相互作用伙伴。利用电子冷冻显微镜，我们提出了全长内亲和素及其N-末端N-bar结构域在其膜结合状态下的重建。内亲素晶格暴露了大片的膜表面，并通过内亲素的两亲性N-末端螺旋之间的混杂相互作用而结合在一起。粗粒分子动力学模拟表明，亲内素晶格是高度动态的，N-末端螺旋是形成稳定而规则的支架所必需的。此外，内亲和素通过量化添加或移除内亲和素二聚体来适应不同的曲率，这在某些情况下会导致内亲亲素的SH3结构域的二聚化，这表明SH3-结构域的空间呈现而不是亲和力控制下游相互作用伙伴的招募。
Functioning as key players in cellular regulation of membrane curvature, BAR-domain proteins bend bilayers and recruit interaction partners through poorly understood mechanisms. Using electron cryomicroscopy, we present reconstructions of full-length endophilin and its N-terminal N-BAR domain in their membrane-bound state. Endophilin lattices expose large areas of membrane surface, and are held together by promiscuous interactions between endophilin's amphipathic N-terminal helices. Coarse-grained molecular dynamics simulations reveal that endophilin lattices are highly dynamic, and that the N-terminal helices are required for formation of a stable and regular scaffold. Furthermore, endophilin accommodates different curvatures through a quantized addition or removal of endophilin dimers, which in some cases causes dimerization of endophilin's SH3 domains, suggesting that the spatial presentation of SH3-domains rather than affinity governs the recruitment of downstream interaction partners.
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影响因子：
64.5
作者：
Hurley JH;Boura E;Carlson LA;Różycki B
通讯作者：
Różycki B
影响因子：
11.8
作者：
Chang-Ileto, Belle;Frere, Samuel G.;Chan, Robin B.;Voronov, Sergey V.;Roux, Aurelian;Di Paolo, Gilbert
通讯作者：
Di Paolo, Gilbert
影响因子：
4.8
作者：
Jao, Christine C.;Hegde, Balachandra G.;Langen, Ralf
通讯作者：
Langen, Ralf
影响因子：
3
作者：
Sorzano, COS;Marabini, R;Pascual-Montano, A
通讯作者：
Pascual-Montano, A
DOI：
10.1042/bss0720223
发表时间：
2005-01-01
期刊：
LIPIDS, RAFTS AND TRAFFIC
影响因子：
--
作者：
Gallop, JL;McMahon, HT
通讯作者：
McMahon, HT