The effect of 3D hydrogel scaffold modulus on osteoblast differentiation and mineralization revealed by combinatorial screening.
The effect of 3D hydrogel scaffold modulus on osteoblast differentiation and mineralization revealed by combinatorial screening.
复制标题
3D水凝胶支架模量对组合细胞分化和矿化的影响，通过组合筛选揭示了。
DOI：
10.1016/j.biomaterials.2010.03.024
复制
发表时间：
2010-07
期刊：
影响因子：
14
通讯作者：
Simon CG Jr
中科院分区：
文献类型：
--
作者：
Chatterjee K;Lin-Gibson S;Wallace WE;Parekh SH;Lee YJ;Cicerone MT;Young MF;Simon CG Jr
已知细胞能够感知并响应其环境和组织支架的物理特性。优化这些细胞-材料相互作用在组织工程中至关重要。在这项工作中，一个简单的和廉价的组合平台，以快速筛选三维（3D）组织支架，并应用于筛选支架性能的影响，组织工程骨。在压缩模量为30倍的聚（乙二醇）水凝胶梯度（10 kPa至1300 kPa）中检查成骨细胞的分化。结果表明，支架的材料特性（凝胶刚度）可以被利用来诱导细胞分化在3D培养作为替代生化线索，如可溶性补充剂，固定的生物分子和载体，这往往是昂贵的，不稳定的和潜在的致癌性。凝胶模量≥ 225 kPa和更高时促进成骨。此外，有人提出，可以调节材料诱导的细胞分化，以设计矿化骨组织和较软组织（如韧带和肌腱）之间的无缝组织界面。这项工作提出了一种组合方法来筛选对3D水凝胶支架的生物反应，该支架更接近地模拟细胞在体内经历的3D环境。
Cells are known to sense and respond to the physical properties of their environment and those of tissue scaffolds. Optimizing these cell-material interactions is critical in tissue engineering. In this work, a simple and inexpensive combinatorial platform was developed to rapidly screen three-dimensional (3D) tissue scaffolds and was applied to screen the effect of scaffold properties for tissue engineering of bone. Differentiation of osteoblasts was examined in poly(ethylene glycol) hydrogel gradients spanning a 30-fold range in compressive modulus (≈ 10 kPa to ≈ 300 kPa). Results demonstrate that material properties (gel stiffness) of scaffolds can be leveraged to induce cell differentiation in 3D culture as an alternative to biochemical cues such as soluble supplements, immobilized biomolecules and vectors, which are often expensive, labile and potentially carcinogenic. Gel moduli of ≈ 225 kPa and higher enhanced osteogenesis. Furthermore, it is proposed that material-induced cell differentiation can be modulated to engineer seamless tissue interfaces between mineralized bone tissue and softer tissues such as ligaments and tendons. This work presents a combinatorial method to screen biological response to 3D hydrogel scaffolds that more closely mimics the 3D environment experienced by cells in vivo.
登录
查看更多内容
影响因子：
6.2
作者：
Lin-Gibson, S;Bencherif, S;Washburn, NR
通讯作者：
Washburn, NR
DOI：
10.2741/beaulieu
发表时间：
1999-03-15
期刊：
Frontiers in Bioscience
影响因子：
--
作者：
Beaulieu, Jean-Francois
通讯作者：
Beaulieu, Jean-Francois
DOI：
10.1073/pnas.0712150105
发表时间：
2008-06-10
影响因子：
11.1
作者：
Moffat, Kristen L.;Sun, Wan-Hsuan S.;Lu, Helen H.
通讯作者：
Lu, Helen H.
影响因子：
48
作者：
Fischbach, Claudia;Chen, Ruth;Mooney, David J.
通讯作者：
Mooney, David J.
影响因子：
5.5
作者：
Khatiwala, CB;Peyton, SR;Putnam, AJ
通讯作者：
Putnam, AJ