Androgenic and oestrogenic influences on tyrosine hydroxylase-immunoreactive cells of the prairie vole medial amygdala and bed nucleus of the stria terminalis.
Androgenic and oestrogenic influences on tyrosine hydroxylase-immunoreactive cells of the prairie vole medial amygdala and bed nucleus of the stria terminalis.
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DOI：
10.1111/j.1365-2826.2010.01958.x
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发表时间：
2010-04
影响因子：
3.2
通讯作者：
Lonstein JS
中科院分区：
文献类型：
--
作者：
Cavanaugh BL;Lonstein JS
杏仁背内侧核(MeApd)和终纹床核主核(PBST)是许多啮齿类动物社会性行为所必需的整合类固醇激素和嗅觉信息的紧密相连的部位。我们实验室最近报道了草原田鼠(Microtus Ochrogaster)MeApd和PBST中含有酪氨酸羟化酶(TH)的性别二型性细胞群，雄性在这些部位拥有比雌性多得多的TH免疫反应(TH-ir)细胞。我们还发现，成年期间循环的性腺激素调节这种性别差异，因为这种差异可以通过去势成年男性或植入充满睾丸激素的胶囊来消除。在这里，我们证明了成年处女草原田鼠的MeApd和PBST中的许多(25%-65%)的TH-ir细胞也含有雄激素受体(AR)或雌激素受体α(ERα)的免疫反应。皮下植入苯甲酸雌二醇酯(EB)模拟了睾酮(T)的作用，并在去势男性的这些部位保持了大量的TH-IR细胞。然而，植入双氢睾丸酮(DHT)并没有，这些雄性动物的TH-ir细胞数量很少，类似于给予空胶囊的去势雄性动物。在女性中也发现了类似的影响，与正常或摘除卵巢的对照组相比，T或EB显著增加了这些部位的TH-ir细胞的数量，但DHT并非如此。然而，DHT植入物确实在男性体内保持了较高的精囊重量。因此，草原田鼠MeApd和PBST中的许多TH-ir细胞对雄激素和雌激素具有潜在的敏感性，但在这些细胞中维持可检测到的TH水平可能更多地依赖于雌性激素介导的机制。这些数据对于理解生殖周期中性腺激素的释放如何调节草原田鼠特定物种的儿茶酚胺能细胞群和社会一夫一妻制行为具有重要意义。
The posterodorsal medial amygdala (MeApd) and principal nucleus of the bed nucleus of the stria terminalis (pBST) are densely interconnected sites integrating steroid hormone and olfactory information necessary for sociosexual behaviours in many rodents. Our laboratory recently reported sexually dimorphic populations of cells containing tyrosine hydroxylase (TH) located in the MeApd and pBST of prairie voles (Microtus ochrogaster), with males having many more TH-immunoreactive (TH-ir) cells in these sites than do females. We also found that gonadal hormones circulating during adulthood regulated this sex difference, as it was eliminated by castrating adult males or implanting females with testosterone-filled capsules. We here demonstrate that many (25-65%) of TH-ir cells in the MeApd and pBST of both sexes of adult virgin prairie voles also contain immunoreactivity for either the androgen receptor (AR) or oestrogen receptor alpha (ERα). Subcutaneous implants of oestradiol benzoate (EB) mimicked the effects of testosterone (T) and maintained high numbers of TH-ir cells in these sites in castrated males. However, implants of dihydrotestosterone (DHT) did not, and these males had low numbers of TH-ir cells similar to castrated males given empty capsules. A similar effect was found in females, where T or EB greatly increased the number of TH-ir cells in these sites compared to intact or ovariectomized controls, but DHT did not. DHT implants did maintain high seminal vesicle weights in males, though. Thus, many of the TH-ir cells in the prairie vole MeApd and pBST are potentially sensitive to androgens and oestrogens, but maintaining immunocytochemically detectable levels of TH in these cells may depend more on an oestrogen-mediated mechanism in both sexes. These data have implications for understanding how gonadal hormone release across the reproductive cycle modulates species-specific groups of catecholaminergic cells and socially monogamous behaviours in prairie voles.
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影响因子：
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作者：
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通讯作者：
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