Comparing Metabolomics Profiles in Various Types of Liquid Biopsies among Screening Participants with and without Advanced Colorectal Neoplasms.
Comparing Metabolomics Profiles in Various Types of Liquid Biopsies among Screening Participants with and without Advanced Colorectal Neoplasms.
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DOI：
10.3390/diagnostics11030561
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发表时间：
2021-03-20
期刊：
影响因子：
--
通讯作者：
Brenner H
中科院分区：
文献类型：
--
作者：
Erben V;Poschet G;Schrotz-King P;Brenner H
代谢组学分析已被认为是早期发现结直肠癌和晚期腺瘤的一种有前途的方法。我们调查和比较了筛查结肠镜检查参与者的血液、粪便和尿样中的代谢组学特征，目的是评估晚期结直肠肿瘤患者和非肿瘤患者之间代谢物浓度的差异。使用MxP®Quant 500试剂盒(奥地利因斯布鲁克的Bicrates)对400名结肠镜筛查参与者的各种类型的生物样本(血浆、粪便和尿液)进行了调查。我们在血液、粪便和尿样中检测到了广泛的代谢物(分别为504、331和131)。93、154和102种代谢物在血和大便、血和尿、粪便和尿液中的浓度之间存在显著相关性，其中68种(73%)、126种(82%)和39种(38%)呈正相关。我们发现患有和不患有晚期结直肠肿瘤的受试者在血液、粪便和尿样中分别有123、49和28种代谢物浓度的显著差异。我们检测到血液样本和尿样或粪便样本的代谢物浓度之间大部分呈正相关，而尿样和粪便样本之间的代谢物浓度大多呈负相关。在患有和不患有晚期结直肠肿瘤的受试者中，发现三种生物体液中的代谢物浓度各不相同。
Analysis of metabolomics has been suggested as a promising approach for early detection of colorectal cancer and advanced adenomas. We investigated and compared the metabolomics profile in blood, stool, and urine samples of screening colonoscopy participants and aimed to evaluate differences in metabolite concentrations between people with advanced colorectal neoplasms and those without neoplasms. Various types of bio-samples (plasma, feces, and urine) from 400 participants of screening colonoscopy were investigated using the MxP® Quant 500 kit (Biocrates, Innsbruck, Austria). We detected a broad range of metabolites in blood, stool, and urine samples (504, 331, and 131, respectively). Significant correlations were found between concentrations in blood and stool, blood and urine, and stool and urine for 93, 154, and 102 metabolites, of which 68 (73%), 126 (82%), and 39 (38%) were positive correlations. We found significant differences between participants with and without advanced colorectal neoplasms for concentrations of 123, 49, and 28 metabolites in blood, stool and urine samples, respectively. We detected mostly positive correlations between metabolite concentrations in blood samples and urine or stool samples, and mostly negative correlations between urine and stool samples. Differences between subjects with and without advanced colorectal neoplasms were found for metabolite concentrations in each of the three bio-fluids.
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影响因子：
3.7
作者：
Breier M;Wahl S;Prehn C;Fugmann M;Ferrari U;Weise M;Banning F;Seissler J;Grallert H;Adamski J;Lechner A
通讯作者：
Lechner A
影响因子：
5.9
作者：
Brown DG;Rao S;Weir TL;O'Malia J;Bazan M;Brown RJ;Ryan EP
通讯作者：
Ryan EP
影响因子：
28.2
作者：
Cantor JR;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
7.1
作者：
Liesenfeld, David B.;Grapov, Dmitry;Ulrich, Cornelia M.
通讯作者：
Ulrich, Cornelia M.
影响因子：
13.6
作者：
DeBerardinis RJ;Chandel NS
通讯作者：
Chandel NS