A rabbit model for evaluating ocular damage from acrolein toxicity in vivo.
A rabbit model for evaluating ocular damage from acrolein toxicity in vivo.
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DOI：
10.1111/nyas.14514
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发表时间：
2020-11
影响因子：
5.2
通讯作者：
Mohan, Rajiv R.
中科院分区：
文献类型：
--
作者：
Gupta, Suneel;Fink, Michael K.;Martin, Lynn M.;Sinha, Prashant R.;Rodier, Jason T.;Sinha, Nishant R.;Hesemann, Nathan P.;Chaurasia, Shyam S.;Mohan, Rajiv R.
丙烯醛是一种高度反应性和挥发性的不饱和醛，通常用于生产数十种商业产品。自第一次世界大战期间使用以来，它一直被认为是一种化学武器，最近在叙利亚使用。丙烯醛的暴露会导致严重的眼睛、皮肤和肺损伤，此外还会造成许多人员伤亡。在眼睛里，它会引起剧烈的疼痛、眼睑肿胀、角膜灼伤和视力障碍。关于丙烯醛如何损害角膜并导致视力丧失的信息非常少。目前，缺乏临床相关的动物模型，限制了丙烯醛毒性的评估和机制，具体到眼睛。本研究旨在规范丙烯醛的给药方式和暴露时间，建立丙烯醛致兔眼损伤的动物模型，为丙烯醛的毒性研究和医学对策的制定提供实验依据。将兔眼暴露于两种递送模式（局部和蒸气）不同的持续时间（1-5分钟）。在长达4周的不同时间对活兔进行裂隙灯、立体显微镜、荧光素染料、角膜厚度计、眼压计和泪液检查的临床眼科检查。组织学诊断角膜透明、纤维化、胶原和新生血管形成。我们的研究成功地建立了一个体内兔模型，用于评估丙烯醛对眼睛的毒性，考虑不同的暴露模式和持续时间。
Acrolein is a highly reactive and volatile unsaturated aldehyde commonly used for producing scores of commercial products. It has been recognized as a chemical weapon since its use during World War I, and more recently, in Syria. Acrolein exposure causes severe eye, skin, and lung damage in addition to many casualties. In the eye, it causes severe pain, eyelid swelling, corneal burns, and vision impairment. Very little information is available about how acrolein damages the cornea and causes vision loss. At present, the lack of clinically relevant animal models limits evaluation of acrolein toxicity and mechanisms specific to the eye. We aim to standardize the mode of delivery and exposure duration of acrolein, damaging the rabbit eye in vivo as an ocular injury model for studying the toxicity of acrolein and developing medical countermeasures. Rabbit eyes were exposed to two modes of delivery (topical and vapor) for different durations (1–5 minutes). Clinical ophthalmic examinations with a slit lamp, stereomicroscope, fluorescein dye, pachymeter, tonometer, and tearing examinations in live rabbits were performed at various times up to 4 weeks. Corneas were histologically diagnosed for transparency, fibrosis, collagens, and neovascularization. Our study successfully established an in vivo rabbit model for evaluating acrolein toxicity to the eye, accounting for different modes and durations of exposure.
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