Functional Analysis of ScABI3 from Syntrichia caninervis Mitt. in Medicago sativa L.

Functional Analysis of ScABI3 from Syntrichia caninervis Mitt. in Medicago sativa L.
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Syntrichia caninervis Mitt 的 ScABI3 功能分析。

DOI:
10.3390/agronomy12092238
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发表时间:
2022-09
期刊:
Agronomy
影响因子:
--
通讯作者:
Yigong Zhang
Yigong Zhang
中科院分区:
其他
文献类型:
--
作者:
Yi Zhang;Chun Wang;Mingqi Huang;Yigong Zhang

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ABI3 (ABSCISIC ACID INSENSITIVE 3) is a family of B3 transcription factors essential for regulating the abscisic acid (ABA) signaling pathway involved in various biological processes and abiotic stress. Our previous studies demonstrated that ectopic expression of ScABI3 from a desiccation-tolerant moss (Syntrichia caninervis) into Arabidopsis thaliana enhanced abiotic stress tolerance. However, studies on plant transformation using the ABI3 gene are limited and other possible functions of ScABI3 are not known. Here, we transformed the ScABI3 into alfalfa (Medicago sativa L.) and analyzed the effects on phenotype, photosynthetic efficiency, and nutritional quality. The results showed that the endogenous ABA content of the transgenic plants was significantly higher than WT, and the leaf-stem ratio, leaf area, and branch number increased with ScABI3 overexpression in alfalfa. Further analysis of the gas exchange parameters showed that the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), and water-use efficiency (WUE) of the transgenic alfalfa were significantly higher than WT; meanwhile, the protein content of the transgenic lines was higher than the WT, but the crude fat content was lower. Thus, these findings suggest that ScABI3 can be used as a potential candidate gene to manipulate alfalfa’s growth and nutritional quality. This study will provide a theoretical basis for breeding alfalfa varieties and assist in forage production and animal husbandry in the future.
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