GmMYB181, a Soybean R2R3-MYB Protein, Increases Branch Number in Transgenic Arabidopsis.
GmMYB181, a Soybean R2R3-MYB Protein, Increases Branch Number in Transgenic Arabidopsis.
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GmMYB181 是一种大豆 R2R3-MYB 蛋白，可增加转基因拟南芥的分枝数量。
DOI：
10.3389/fpls.2018.01027
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发表时间：
2018
影响因子：
5.6
通讯作者：
Huang F
中科院分区：
文献类型：
--
作者：
Yang H;Xue Q;Zhang Z;Du J;Yu D;Huang F
大豆是一种重要的经济作物，为人类提供丰富的油脂和优质蛋白质。由于生殖生长过程直接决定了作物种子的产量和质量，我们启动了对调控大豆花器官发育的基因的研究。通过基因芯片分析，发现一个R2R3-MYB转录因子基因GmMYB181在花中丰富，并在转基因拟南芥中进行了进一步的功能研究。GmMYB181蛋白含有两个MYB结构域，定位于细胞核，在酵母杂交系统中表现出转录活性。实时定量聚合酶链式反应(qRT-PCR)结果表明，GmMYB181仅在花组织中表达。在拟南芥中，GmMYB181基因的过表达改变了花器官的形态、果实大小和植株结构，包括向外卷曲的萼片、较小的角果、增加侧枝和降低株高，表明GmMYB181参与生殖器官的发育并在植物结构控制中发挥重要作用。此外，基因芯片分析表明，在拟南芥中过表达GmMYB181影响成熟花中3450个基因的表达，包括那些与花器官、种子/果实发育有关的基因，并对不同的激素信号做出反应。
Soybean (Glycine max) is an important economic crop that provides abundant oil and high quality protein for human beings. As the process of reproductive growth directly determines the crop seed yield and quality, we initiated studies to identify genes that regulate soybean floral organ development. One R2R3-MYB transcription factor gene, designated as GmMYB181, was found to be enriched in flowers based on microarray analysis and was further functionally investigated in transgenic Arabidopsis. GmMYB181 protein contains two MYB domains, which localized to the nucleus and displayed transcriptional activation in yeast hybrid system. Real-time quantitative PCR (qRT-PCR) results suggested GmMYB181 exclusively expressed in flower tissue. In Arabidopsis, overexpression of GmMYB181 altered the morphology of floral organs, fruit size and plant architecture, including outward curly sepals, smaller siliques, increased lateral branches and reduced plant height, indicating that GmMYB181 is involved in the development of reproductive organs and plays an important role in controlling plant architecture. Further, microarray analysis revealed that overexpressing GmMYB181 in Arabidopsis affected the expression of 3450 genes in mature flowers, including those involved in floral organ, seed/fruit development, and responded to different hormone signals.
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