Comparative analysis of humoral immune responses and pathologies of BALB/c and C57BL/6 wildtype mice experimentally infected with a highly virulent Rodentibacter pneumotropicus (Pasteurella pneumotropica) strain.
Comparative analysis of humoral immune responses and pathologies of BALB/c and C57BL/6 wildtype mice experimentally infected with a highly virulent Rodentibacter pneumotropicus (Pasteurella pneumotropica) strain.
复制标题
DOI：
10.1186/s12866-018-1186-8
复制
发表时间：
2018-05-30
期刊：
影响因子：
4.2
通讯作者：
Baums CG
中科院分区：
文献类型：
--
作者：
Fornefett J;Krause J;Klose K;Fingas F;Hassert R;Benga L;Grunwald T;Müller U;Schrödl W;Baums CG
小鼠是吸肺啮齿类细菌的天然宿主。尽管有专门的监测，但它仍然是实验室动物中最重要的传染性病原体之一。本研究的目的是在一种新的动物模型中确定一种流行的肺吸虫病型的毒力并表征宿主的反应。C57BL/6和BALB/c小鼠鼻内感染携带毒素（RTX）毒素中三个已知重复序列基因的肺嗜肺孢子虫菌株（JF4Ni），死亡率和发病率分别高达50%和80%以上。发病率与严重的体重减轻以及结膜炎和呼吸困难有关。主要病理为卡他性化脓性至坏死性支气管肺炎。在大多数存活小鼠的气管鼻灌洗液中检测到特异性免疫球蛋白（Ig） A。此外，所有存活的动物都显示出不同的IgG抗体产生。为了区分辅助性t细胞（Th） 1和Th2免疫应答，我们使用igg亚类作为指标。感染前和感染后BALB/c小鼠血清中IgG2b与IgG1的平均比值均低于0.8。相比之下，C57BL/6小鼠感染后的平均IgG2b/IgG1比值为1.6，表明C57BL/6小鼠的Th1免疫应答与BALB/c小鼠的Th2应答相关，其肺部细菌负荷高10倍。根据Th1反应，在大多数存活的C57BL/6小鼠中检测到高抗原特异性IgG2c滴度。嗜肺孢子虫JF4Ni是一种高毒力菌株，在C57BL/6和BALB/c小鼠鼻内感染后可引起严重肺炎和败血症。两种小鼠株的持续感染分别与Th1和Th2免疫反应以及肺部细菌负荷的差异有关。所描述的模型非常适合未来使用自然宿主的疫苗接种研究。本文的在线版本（10.1186/s12866-018-1186-8）包含补充材料，授权用户可以使用。
Mice are a natural host for Rodentibacter (R.) pneumotropicus. Despite specific monitoring, it is still one of the most important infectious agents in laboratory animals. The objective of this study was to determine the virulence of a prevalent pathotype of R. pneumotropicus and characterize the host response in a new animal model. Intranasal infection of C57BL/6 and BALB/c mice with a R. pneumotropicus strain (JF4Ni) bearing the genes of the three known repeats in toxin (RTX) toxins resulted in an unprecedented high mortality and morbidity above 50 and 80%, respectively. Morbidity was associated with severe weight loss as well as conjunctivitis and dyspnea. A main pathology was a catarrhal purulent to necrotic bronchopneumonia. Specific immune globuline (Ig) A was detected in tracheonasal lavages of most surviving mice which were still colonized by R. pneumotropicus. Furthermore, all surviving animals showed a distinct production of IgG antibodies. To differentiate T-helper cell (Th) 1 and Th2 immune responses we used subclasses of IgGs as indicators. Mean ratios of IgG2b to IgG1 were below 0.8 in sera drawn from both mice strains prior infection and from BALB/c mice post infection. In contrast, C57BL/6 mice had a mean IgG2b/IgG1 ratio of 1.6 post infection indicating a Th1 immune response in C57BL/6 versus a Th2 response in BALB/c mice associated with a tenfold higher bacterial load in the lung. In accordance with a Th1 response high antigen-specific IgG2c titers were detected in the majority of surviving C57BL/6 mice. R. pneumotropicus JF4Ni is a highly virulent strain causing severe pneumonia and septicemia after intranasal infection of C57BL/6 and BALB/c mice. Persisting infections in the two mice strains are associated with Th1 and Th2 immune responses, respectively, and differences in the bacterial burden of the lung. The described model is ideally suited for future vaccination studies using the natural host. The online version of this article (10.1186/s12866-018-1186-8) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
2.2
作者：
Benga, Laurentiu;Benten, W. Peter M.;Sager, Martin
通讯作者：
Sager, Martin
影响因子：
2.4
作者：
BOOT, R;THUIS, HCW;BAKKER, RGH
通讯作者：
BAKKER, RGH
影响因子：
4.2
作者：
Sasaki, Hiraku;Ishikawa, Hiroki;Shirama, Kazuhiko
通讯作者：
Shirama, Kazuhiko
影响因子：
3.2
作者：
Sasaki, Hiraku;Kawamoto, Eiichi;Yagami, Ken-ichi
通讯作者：
Yagami, Ken-ichi
影响因子：
2.2
作者：
Martin, RM;Brady, JL;Lew, AM
通讯作者：
Lew, AM