The SWEET gene family in Hevea brasiliensis - its evolution and expression compared with four other plant species.
The SWEET gene family in Hevea brasiliensis - its evolution and expression compared with four other plant species.
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巴西橡胶树的 SWEET 基因家族 - 与其他四种植物相比的进化和表达
DOI：
10.1002/2211-5463.12332
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发表时间：
2017-12
期刊：
影响因子：
2.6
通讯作者：
Tang CR
中科院分区：
文献类型：
--
作者：
Sui JL;Xiao XH;Qi JY;Fang YJ;Tang CR
SWEET蛋白作为糖外排转运蛋白在植物发育和逆境应答中起着不可或缺的作用。SWEET基因先前已经在几种植物中被表征。在这里，我们提出了一个全面的分析这个基因家族在橡胶树，橡胶树。该物种中有36个SWEET基因家族成员，使其成为迄今为止测序的植物基因组中最大的家族之一。这些基因在橡胶树和其他物种的结构和遗传分析表明，广泛的进化保守。RNA-seq分析表明，SWEET 2、16和17可能代表了SWEET基因在植物中的主要进化方向。我们在橡胶树中的结果表明HbSWEET 1a，2 e，2f和3b参与韧皮部装载，HbSWEET 10a和16 b参与乳管糖运输，HbSWEET 9a参与蜜腺特异性糖运输。RNA测序分析的平行研究扩展到其他三种植物物种（Manihot esculenta，Populus ducarpa和Arabidopsis thaliana），结果表明MeSWEET 10a，3a和15 b在M.结果表明，PtSWEET 16 b和PtSWEET 16 d参与了番茄贮藏根的发育，PtSWEET 16 b和PtSWEET 16 d参与了番茄木质部发育。RT-qPCR结果进一步揭示了HbSWEET 10a、16 b和1a在韧皮部糖运输中起重要作用。本研究结果不仅为进一步研究橡胶树中SWEET基因家族的功能，特别是其在胶乳生产中的糖转运功能奠定了基础，而且也为该基因家族在植物界的相关研究奠定了基础。
SWEET proteins play an indispensable role as a sugar efflux transporter in plant development and stress responses. The SWEET genes have previously been characterized in several plants. Here, we present a comprehensive analysis of this gene family in the rubber tree, Hevea brasiliensis. There are 36 members of the SWEET gene family in this species, making it one of the largest families in plant genomes sequenced so far. Structure and phylogeny analyses of these genes in Hevea and in other species demonstrated broad evolutionary conservation. RNA‐seq analyses revealed that SWEET2, 16, and 17 might represent the main evolutionary direction of SWEET genes in plants. Our results in Hevea suggested the involvement of HbSWEET1a, 2e, 2f, and 3b in phloem loading, HbSWEET10a and 16b in laticifer sugar transport, and HbSWEET9a in nectary‐specific sugar transport. Parallel studies of RNA‐seq analyses extended to three other plant species (Manihot esculenta, Populus trichocarpa, and Arabidopsis thaliana) produced findings which implicated MeSWEET10a, 3a, and 15b in M. esculenta storage root development, and the involvement of PtSWEET16b and PtSWEET16d in P. trichocarpa xylem development. RT‐qPCR results further revealed that HbSWEET10a, 16b, and 1a play important roles in phloem sugar transport. The results from this study provide a foundation not only for further investigation into the functionality of the SWEET gene family in Hevea, especially in its sugar transport for latex production, but also for related studies of this gene family in the plant kingdom.
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影响因子：
4.6
作者：
Lau NS;Makita Y;Kawashima M;Taylor TD;Kondo S;Othman AS;Shu-Chien AC;Matsui M
通讯作者：
Matsui M
影响因子：
4.4
作者：
Rahman AY;Usharraj AO;Misra BB;Thottathil GP;Jayasekaran K;Feng Y;Hou S;Ong SY;Ng FL;Lee LS;Tan HS;Sakaff MK;Teh BS;Khoo BF;Badai SS;Aziz NA;Yuryev A;Knudsen B;Dionne-Laporte A;Mchunu NP;Yu Q;Langston BJ;Freitas TA;Young AG;Chen R;Wang L;Najimudin N;Saito JA;Alam M
通讯作者：
Alam M
影响因子：
4.4
作者：
Liu JP;Xia ZQ;Tian XY;Li YJ
通讯作者：
Li YJ
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影响因子：
7.3
作者：
Liu, Qinsong;Yuan, Meng;Wang, Shiping
通讯作者：
Wang, Shiping
影响因子：
1.8
作者：
Chow KS;Ghazali AK;Hoh CC;Mohd-Zainuddin Z
通讯作者：
Mohd-Zainuddin Z