Transcriptome sequencing and analysis of rubber tree (Hevea brasiliensis Muell.) to discover putative genes associated with tapping panel dryness (TPD).
Transcriptome sequencing and analysis of rubber tree (Hevea brasiliensis Muell.) to discover putative genes associated with tapping panel dryness (TPD).
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DOI：
10.1186/s12864-015-1562-9
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发表时间：
2015-05-21
期刊：
影响因子：
4.4
通讯作者：
Li YJ
中科院分区：
文献类型：
--
作者：
Liu JP;Xia ZQ;Tian XY;Li YJ
割胶板干燥（TPD）会导致乳胶流动部分或完全停止，从而严重影响橡胶树（Hevea brasiliensis）的乳胶生产。已经进行了大量研究来确定其起源和性质，但 TPD 发生的分子性质和机制仍不清楚。本研究致力于对健康（H）和受TPD影响（T）橡胶树的转录组进行从头测序和比较分析，以鉴定与TPD相关的基因和通路。使用 Illumina Hiseq 2000 测序技术从 H 和 T 文库中分别获得了 34,632,012 和 35,913,020 bp 的总原始读数。从头组装分别从 H 和 T 文库中产生了 141,456 和 169,285 个重叠群，以及 96,070 和 112,243 个 unigene。在73597个基因中，通过比较转录谱分析，22577个基因被鉴定为H和T文库之间的差异表达基因。发现与 TPD 发生最有潜在相关性的大多数涉及天然橡胶生物合成和茉莉酸合成的基因存在差异表达。在受TPD影响的树木中，大多数与乳胶生物合成和茉莉酸合成相关的基因的表达受到严重抑制，这可能是TPD的直接原因。这些新的从头转录组数据集为发现 TPD 相关基因提供了重要资源，并提高了我们对 TPD 发生和维持的理解。本文的在线版本 (doi:10.1186/s12864-015-1562-9) 包含补充材料，可供授权用户使用。
Tapping panel dryness (TPD) involves in the partial or complete cessation of latex flow thus seriously affect latex production in rubber tree (Hevea brasiliensis). Numerous studies have been conducted to define its origin and nature, but the molecular nature and mechanism of TPD occurrence remains unknown. This study is committed to de novo sequencing and comparative analysis of the transcriptomes of healthy (H) and TPD-affected (T) rubber trees to identify the genes and pathways related to the TPD. Total raw reads of 34,632,012 and 35,913,020 bp were obtained from H and T library, respectively using Illumina Hiseq 2000 sequencing technology. De novo assemblies yielded 141,456 and 169,285 contigs, and 96,070 and 112,243 unigenes from H and T library, respectively. Among 73597 genes, 22577 genes were identified as differential expressed genes between H and T library via comparative transcript profiling. A majority of genes involved in natural rubber biosynthesis and jasmonate synthesis with most potential relevance in TPD occurrence were found to be differentially expressed. In TPD-affected trees, the expression of most genes related to the latex biosynthesis and jasmonate synthesis was severely inhibited and is probably the direct cause of the TPD. These new de novo transcriptome data sets provide a significant resource for the discovery of genes related to TPD and improve our understanding of the occurrence and maintainace of TPD. The online version of this article (doi:10.1186/s12864-015-1562-9) contains supplementary material, which is available to authorized users.
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影响因子：
7.8
作者：
Chang, Wei-chen;Song, Heng;Liu, Hung-wen;Lu, Pinghua
通讯作者：
Lu, Pinghua
DOI：
10.1016/s0044-328x(84)80020-3
发表时间：
1984-01-01
期刊：
ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE
影响因子：
--
作者：
CHRESTIN, H;BANGRATZ, J;JACOB, JL
通讯作者：
JACOB, JL
影响因子：
3.8
作者：
da Costa, Bernardo M. T.;Keasling, Jay D.;Cornish, Katrina
通讯作者：
Cornish, Katrina
影响因子：
4.2
作者：
Hao, BZ;Wu, JL
通讯作者：
Wu, JL
影响因子：
6.9
作者：
Chow KS;Mat-Isa MN;Bahari A;Ghazali AK;Alias H;Mohd-Zainuddin Z;Hoh CC;Wan KL
通讯作者：
Wan KL