Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol.
Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol.
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DOI：
10.1016/j.neuroscience.2014.07.066
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发表时间：
2014-10-10
期刊：
影响因子：
3.3
通讯作者：
Bellinger, L. L.
中科院分区：
文献类型：
--
作者：
Kramer, P. R.;Bellinger, L. L.
高水平的17β-雌二醇（E2）已被发现可以减轻炎症性颞下颌关节（TMJ）疼痛。对受高浓度雌二醇影响的基因的研究表明，三叉神经节（TG）中GABAA受体亚基α6 （Gabrα6）升高。阻断TG中Gabrα6的表达会增加雄性大鼠咬肌痛觉，但雌二醇对雌性大鼠痛觉的影响与Gabrα6表达之间的关系尚不清楚。为了解决这一知识差距，我们假设降低TG中Gabrα6的表达会增加雌二醇治疗的去卵巢雌性大鼠的口面部伤害反应。为了给药，我们在大鼠体内放置渗透泵，给药后低浓度的17β-雌二醇，另外，通过注射17β-雌二醇使大鼠产生高浓度的雌二醇。然后在咬肌肌腱周围打结以诱导伤害性反应；TMJ肌肉疼痛模型随后将Gabrα6 siRNA注入TG，并使用von Frey细丝和用餐时间法测量伤害反应。测定GABAA受体在TG、三叉神经尾核及颈上区（Vc-C1）的表达。结扎可显著增加痛觉反应，但高发情浓度的17β-雌二醇可减弱这种反应。Gabrα6 siRNA输注降低TG和Vc-C1中Gabrα6的表达，但增加了17β-雌二醇治疗后的伤害反应。结果表明，雌二醇降低了口面部伤害反应，部分原因是引起Gabrα6表达的增加。
High levels of 17β-estradiol (E2) have been found to reduce inflammatory temporomandibular joint (TMJ) pain. A search for genes effected by a high concentration of estradiol showed an increase in GABAA receptor subunit α6 (Gabrα6) in the trigeminal ganglia (TG). Blockade of Gabrα6 expression in the TG increases masseter muscle nociception in male rats, but the relationship between estradiol’s effect on nociception and Gabrα6 expression remains unclear in females. To address this knowledge gap we hypothesized that reducing Gabrα6 expression in the TG will increase the orofacial nociceptive response of ovariectomized female rats treated with estradiol. To administer hormone osmotic pumps were placed in rats that dispensed a low diestrus plasma concentration of 17β-estradiol, in addition, 17β-estradiol was injected to produce a high proestrus plasma concentration of estradiol. A ligature was then placed around the masseter tendon to induce a nociceptive response; a model for TMJ muscle pain. Gabrα6 siRNA was later infused into the TG and the nociceptive response was measured using von Frey filaments and a meal duration assay. GABAA receptor expression was measured in the TG and trigeminal nucleus caudalis and upper cervical region (Vc-C1). Ligature significantly increased the nociceptive response but a high proestrus concentration of 17β-estradiol attenuated this response. Gabrα6 siRNA infusion decreased Gabrα6 expression in the TG and Vc-C1 but increased the nociceptive response after 17β-estradiol treatment. The results suggest estradiol decreased the orofacial nociceptive response, in part, by causing an increase in Gabrα6 expression.
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影响因子：
3.3
作者：
Anderson WB;Graham BA;Beveridge NJ;Tooney PA;Brichta AM;Callister RJ
通讯作者：
Callister RJ
影响因子：
3.6
作者：
Kerins, CA;Carlson, DS;Bellinger, LL
通讯作者：
Bellinger, LL
影响因子：
4.8
作者：
Connolly, CN;Krishek, BJ;Moss, SJ
通讯作者：
Moss, SJ
影响因子：
4.8
作者：
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通讯作者：
Bellinger, L. L.
影响因子：
2.9
作者：
Bellinger, Larry L.;Spears, Robert;Kramer, Phillip R.
通讯作者：
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