A novel functional gene associated with cold tolerance at the seedling stage in rice.
A novel functional gene associated with cold tolerance at the seedling stage in rice.
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DOI：
10.1111/pbi.12704
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发表时间：
2017-09
影响因子：
13.8
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Zhao J;Zhang S;Dong J;Yang T;Mao X;Liu Q;Wang X;Liu B
鉴定和克隆能够在不同寒冷环境下稳定表达的耐寒基因对于水稻耐寒分子育种至关重要。在前期研究中，我们利用耐寒品种丽江新团黑谷（LTH）和冷敏感品种山黄战2号（SHZ-2）的重组自交系（RIL）群体，鉴定了苗期耐寒QTL qCTS-9，该QTL可以在不同寒冷环境下检测到。在本研究中，通过QTL作图与LTH全基因组差异表达谱的综合分析，鉴定了qCTS-9区间内的8个候选基因。 qRT-PCR检测显示，只有Os09g0410300在冷胁迫下LTH和SHZ-2之间表现出不同的表达模式，并且Os09g0410300的冷诱导与RI系中幼苗的耐冷性之间存在显着正相关。在LTH和SHZ-2之间检测到Os09g0410300启动子中的5个SNP和1个InDel，基于启动子插入缺失多态性设计的InDel标记ID410300与RIL群体幼苗的耐寒性显着相关。此外，与野生型植物相比，Os09g0410300过表达植物在苗期表现出增强的耐寒性。因此，我们的结果表明 Os09g0410300 是 qCTS-9 的功能基因。据我们所知，这是一个有助于增强水稻苗期耐寒性的新基因。 qCTS-9功能基因的鉴定和基因特异性标记的开发将有助于通过转基因方法和标记辅助选择（MAS）在水稻苗期进行耐冷分子育种。
Identification and cloning of cold‐tolerant genes that can stably express under different cold environments are crucial for molecular rice breeding for cold tolerance. In the previous study, we identified a cold‐tolerant QTL at the seedling stage, qCTS‐9 which could be detected under different cold environments using a recombinant inbred line (RIL) population derived from a cold‐tolerant variety Lijiangxintuanheigu (LTH) and a cold‐sensitive variety Shanhuangzhan 2 (SHZ‐2). In this study, eight candidate genes within the qCTS‐9 interval were identified through integrated analysis of QTL mapping with genomewide differential expression profiling of LTH. The qRT‐PCR assay showed that only Os09g0410300 exhibited different expression patterns between LTH and SHZ‐2 during cold stress, and significantly positive correlation was found between cold induction of Os09g0410300 and seedling cold tolerance in the RI lines. Five SNPs and one InDel in the promoters of Os09g0410300 were detected between LTH and SHZ‐2, and the InDel marker ID410300 designed based on the insertion–deletion polymorphism in the promoter was significantly associated with seedling cold tolerance in RIL population. Further, Os09g0410300 over‐expression plants exhibited enhanced cold tolerance at the seedling stage compared with the wild‐type plants. Thus, our results suggest that Os09g0410300 is the functional gene underlying qCTS‐9. To our knowledge, it is a novel gene contributed to enhance cold tolerance at the seedling stage in rice. Identification of the functional gene underlying qCTS‐9 and development of the gene‐specific marker will facilitate molecular breeding for cold tolerance at the seedling stage in rice through transgenic approach and marker‐assisted selection (MAS).
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影响因子：
5.6
作者：
Kurepin LV;Dahal KP;Savitch LV;Singh J;Bode R;Ivanov AG;Hurry V;Hüner NP
通讯作者：
Hüner NP
影响因子：
30.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
5.1
作者：
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通讯作者：
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