Discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis.
Discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis.
复制标题
自发性结肠炎小鼠模型中肠道微生物群的变化与致病性之间的不一致。
DOI：
10.4161/gmic.28622
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发表时间：
2014-05
期刊：
影响因子：
12.2
通讯作者：
Peterson DA
中科院分区：
文献类型：
--
作者：
Perez-Muñoz ME;Bergstrom K;Peng V;Schmaltz R;Jimenez-Cardona R;Marsteller N;McGee S;Clavel T;Ley R;Fu J;Xia L;Peterson DA
在常规条件下，缺乏核心1衍生的O-聚糖（TM-IEC C1galt1−/−）的小鼠，其粘液层有缺陷，会经历结肠自发炎症。通过 16S rRNA 基因焦磷酸测序对粪便细菌种群进行分析表明，传统 TM-IEC C1galt1−/− 中的疾病与微生物群的变化相关，具体表现为乳杆菌和梭菌属物种的增加，以及未分类的瘤胃球菌科和毛螺菌科的减少。在无菌（GF）条件下，TM-IEC C1galt1−/− 呈现杯状细胞减少，但未发生炎症。 GF TM-IEC C1galt1−/− 的单关联揭示了细菌种类在诱导炎症变化的能力方面存在显着差异。多形拟杆菌会引起炎症，而约氏乳杆菌（在结肠炎期间富含）则不会。这些观察结果表明，并非所有与疾病相关的微生物群变化都会导致发病机制。
Under conventional conditions, mice deficient in core 1-derived O-glycans (TM-IEC C1galt1−/−), which have a defective mucus layer, experienced spontaneous inflammation of the colon. Analysis of fecal bacterial populations by pyrosequencing of 16S rRNA gene showed that disease in conventional TM-IEC C1galt1−/− was associated with shifts in the microbiota manifested by increases in Lactobacillus and Clostridium species, and decreases in unclassified Ruminococcaceae and Lachnospiraceae. Under germ-free (GF) conditions, TM-IEC C1galt1−/− presented decreased goblet cells, but did not develop inflammation. Monoassociation of GF TM-IEC C1galt1−/− revealed that bacterial species differ significantly in their ability to induce inflammatory changes. Bacteroides thetaiotaomicron caused inflammation, while Lactobacillus johnsonii (enriched during colitis) did not. These observations demonstrate that not all microbiota shifts that correlate with disease contribute to pathogenesis.
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影响因子：
4.6
作者：
Vigsnaes, Louise Kristine;van den Abbeele, Pieter;Sulek, Karolina;Frandsen, Henrik Lauritz;Steenholdt, Casper;Brynskov, Jorn;Vermeiren, Joan;van de Wiele, Tom;Licht, Tine Rask
通讯作者：
Licht, Tine Rask
影响因子：
9.8
作者：
Png, Chin Wen;Linden, Sara K.;Florin, Timothy H. J.
通讯作者：
Florin, Timothy H. J.
影响因子：
56.9
作者：
Bry, L;Falk, PG;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
17.1
作者：
Peterson, Daniel A.;Turnbaugh, Peter J.
通讯作者：
Turnbaugh, Peter J.
DOI：
10.1111/j.1742-1241.2007.01665.x
发表时间：
2008-05-01
影响因子：
2.6
作者：
Strugala, V.;Dettmar, P. W.;Pearson, J. P.
通讯作者：
Pearson, J. P.