Control of lateral migration and germ cell elimination by the Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2.
Control of lateral migration and germ cell elimination by the Drosophila melanogaster lipid phosphate phosphatases Wunen and Wunen 2.
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DOI：
10.1083/jcb.200506038
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发表时间：
2005-11-21
期刊：
影响因子：
--
通讯作者：
Lehmann R
中科院分区：
文献类型：
--
作者：
Sano H;Renault AD;Lehmann R
在大多数生物中，原始生殖细胞(PGCs)出现在远离体细胞性腺前体(SGPs)的区域。虽然生殖细胞通常起源于单个细胞团，但性腺的体细胞部分在胚胎的每个部位形成。因此，为了到达性腺，生殖细胞不仅从它们的起源地迁徙，而且还分裂成两个群体。利用高分辨率实时成像的优势，我们发现在果蝇中，黑腹果蝇的PGCs是极化的，并向SGPS方向迁移，避开了中线。出乎意料的是，既不需要在SGPS中合成的PGC引诱剂，也不需要已知的轴突引导中线驱避剂来对PGC进行双边分类。然而，中枢神经系统表达的wunen(Wun)和wunen-2(wun-2)提供的排斥活性在这种迁移过程中是必不可少的，并控制着PGC的生存。我们的结果表明，Wun/Wun-2排斥剂沿迁移路径的表达提供了对PGCs分选到两个性腺的忠实控制，并消除了留在胚胎中部的PGCs。
In most organisms, primordial germ cells (PGCs) arise far from the region where somatic gonadal precursors (SGPs) are specified. Although PGCs in general originate as a single cluster of cells, the somatic parts of the gonad form on each site of the embryo. Thus, to reach the gonad, PGCs not only migrate from their site of origin but also split into two groups. Taking advantage of high-resolution real-time imaging, we show that in Drosophila melanogaster PGCs are polarized and migrate directionally toward the SGPs, avoiding the midline. Unexpectedly, neither PGC attractants synthesized in the SGPs nor known midline repellents for axon guidance were required to sort PGCs bilaterally. Repellent activity provided by wunen (wun) and wunen-2 (wun-2) expressed in the central nervous system, however, is essential in this migration process and controls PGC survival. Our results suggest that expression of wun/wun-2 repellents along the migratory paths provides faithful control over the sorting of PGCs into two gonads and eliminates PGCs left in the middle of the embryo.
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